TTF-1临床检测诊断pN0期肺腺癌淋巴结微转移  

Detection of TTF-1 for Diagnosis of Lymph Node Micrometastasis at pN_0 Stage of Lung Adenocarcinoma

作　　者：刘宏[1] 叶玉坤[1] 李桂梅[1] 周源[1] 韩开宝[1] 许罡[1] 汪栋[1] 

机构地区：[1]第二军医大学八一医院临床学院,中国人民解放军第81医院,江苏南京210002

出　　处：《中国肿瘤》2012年第4期303-305,共3页China Cancer

基　　金：南京军区医药卫生重点项目(07Z011)

摘　　要：[目的]探讨检测甲状腺转录因子-1(TTF-1)对肺腺癌淋巴结微转移的诊断价值。[方法]选取54例术后常规HE诊断为N0期,且肿瘤原发灶TTF-1表达阳性的肺腺癌病例,采用免疫组化法检测TTF-1在54例患者共893枚淋巴结中的表达。选取同期明确淋巴结转移且原发灶TTF-1表达阳性的肺腺癌病例作为阳性对照,5例肺良性病变术中所取5枚肺内或纵隔淋巴结作为阴性对照。[结果]54例患者893枚淋巴结中检测出9例共21枚淋巴结阳性,阳性淋巴结检出率2.4%(21/893),微转移病例检出率16.7%(9/54)。TTF-1在阳性对照组淋巴结中均表达,在阴性对照组中无表达。[结论]采用免疫组化法检测淋巴结中TTF-1表达,可以发现并诊断肺腺癌淋巴结微转移。[Purpose]To investigate the diagnostic value of detection of thyroid transcription factor-1 （TTF-1） for lymph node micrometastasis in lung adenocarcinomas.[Methods] Fifty-four pN0 stage （node negative according to histologic examination） lung adenocarcinoma patients with positive expression of TFF-I in primary lung adenocarcinoma niduses were involved in this study. The TTF-1 expression in 893 lymph nodes from the patients were detected by immunohistochemical method. The metastasis lymph nodes from lung adenocarcinoma patients with the positive expression of TI＇F-I in primary niduses were selected as positive control, 5 lymph nodes from 5 patients of lung benign lesion were se- lected as negative control. [Results ]Among 893 lymph node samples from 54 patients, 21 lymph node samples from 9 patients was found positive. The rate of positive lymph node was 2.4%. The rate of the patients with micrometastasis in lymph node was 16.7%. TTF-1 expressed in all of lymph nodes of the postive control group, none of lymph node showed TTF-1 expression in negative control group. [Conclusion]The immunohistochemistry assay of TI＇F-I is a potential method for the diagnosis of mi- crometastasis in lymph node of lung adenocarcinoma patients.

关 键 词：肺腺癌 淋巴结微转移 甲状腺转录因子-1 免疫组化 

分 类 号：R735.2[医药卫生—肿瘤]