早期胃印戒细胞癌内肿瘤干细胞分布的病理学研究  

A Pathological Study of the Distribution of Cancer Stem Cells in Early Gastric Signet-Ring Cell Carcinoma

作　　者：张建东[1] 周志华[1] 徐桂芳[2] 赵海滨[1] 赵玲娜[1] 单宝珍[1] 

机构地区：[1]无锡市解放军第101医院,江苏大学教学医院,江苏无锡214044 [2]南京大学医学院附属鼓楼医院,江苏南京210008

出　　处：《中国肿瘤》2012年第4期316-320,共5页China Cancer

基　　金：国家自然科学基金(81000964)

摘　　要：[目的]探讨早期胃印戒细胞癌内肿瘤干细胞的分布和定位。[方法]采用组织化学和免疫组织化学染色技术,检测47例早期胃印戒细胞癌的黏液分泌特点、分化增殖特性和干细胞标志物的表达。[结果]早期胃印戒细胞癌具有特征性的"分层结构":浅表层为典型印戒细胞,第二层为低分化癌细胞,少数病例(4例)含第三层癌细胞,呈泡沫样。浅表层及第三层癌细胞具有黏液分泌功能,主要表达MUC5AC和MUC6,而不表达干细胞标志物。第二层癌细胞无黏液分泌功能,不表达分化标志物,Sox2、musashi-1、hTERT干细胞标记阳性,Ki-67阳性率为28.6%±4.3%。[结论]早期胃印戒细胞癌的肿瘤干细胞定位于分层结构的第二层,可能与肿瘤的早期进展有关。[Purpose] To investigate the distribution and location of cancer stem cells in early gas- tric signet-ring cell carcinoma （SRCC）. [Methods] The characteristics of mucus secretion,differen- tiation and proliferation,and stem cell markers expression in 47 cases with early gastric SRCC were detected by histochemical and irnmunohistochemical staining. [Results] Early gastric SRCC was characterized by a layered structure including three layers： superficial layer,containing typical signet-ring cells; the second layer, containing poorly-differentiated cancer cells; the third layer in few cases （4 cases） containing foamy cells. Most cells in the superficial layer and the third layer with mucus secretion,main expression of MUC5AC and MUC6,and stem cell markers were all negative expression. The second layer without mucus secretion,the differentiation markers were all negative expression,whereas the stem cell markers of Sox2,musashi-1 ,hTERT were positive,and the positive rate of Ki-67 was 28.6%±4.3%. [Conclusion] Cancer stem cells of early gastric SRCC are located at the second layer of the layered structure ,and they might relate to cancer early pro- gression.

关 键 词：胃印戒细胞癌 干细胞 分层结构 免疫组化 

分 类 号：R735.2[医药卫生—肿瘤]