表面等离子体共振生物传感器在卵巢肿瘤标志物CA125检测中的应用  

Application of surface plasmon resonance biosensor in detection of ovarian tumor marker CA125

作　　者：王耀玲 王瑞娟 王亚琴[2] 

机构地区：[1]西安市第二医院妇产科,西安710003 [2]陕西省人民医院妇产科

出　　处：《陕西医学杂志》2012年第2期212-214,共3页Shaanxi Medical Journal

基　　金：西安市科技计划资助项目(SF09028)

摘　　要：目的:运用表面等离子体共振生物传感器(SPR)无标记检测CA125血清抗原,探索卵巢癌无创诊断方法。方法:采用分子自组装技术,在SPR生物传感片表面固定抗CA125单克隆抗体OC125,利用其检测人体血清中CA125抗原的表达。结果:SPR对血清中CA125的测量结果与放射免疫测量结果的变化趋势基本一致,SPR共振峰移动的大小(δλ)相对地反映了血清中CA125抗原表达程度。结论:卵巢癌患者血清的SPR共振峰波长移动值显著大于对照组血清的SPR共振峰波长移动值。与现有检测技术相比,SPR方法具有无需标记,操作简单等优点,便于基层医疗单位使用。Objective：Explore a label free method for CA125 antigen detection in human serum for clinical diagnosis of ovarian cancer.Methods：Surface plasmon resonance biosensor（SPR）was used and 15 case of human sera were measured using SPR method.The specimens were also measured using RIA method for comparison.Results：SPR results were relatively according with that of RIA method.Conclusion：SPR technology can be used as label free method in ovarian cancer marker CA125 detection.

关 键 词：卵巢肿瘤/诊断 CA-125抗原 @表面等离子体共振生物传感器 

分 类 号：R737.31[医药卫生—肿瘤]