微血管密度与宫颈鳞癌组织微淋巴管生成、癌细胞增殖、浸润转移及预后的关系  

Relationship between microvessel density and lymphogenesis,cancer cells proliferation,invasion,metastasis and prognosis in squamous cell carcinoma of cervix

作　　者：齐碧如[1] 江忠清[2] 连成瑛[3] 黄勉[1] 林岷 陈玉华[1] 刘兆董[3] 方娇宁[3] 

机构地区：[1]福州市第一医院妇产科 [2]福建医科大学附属第一医院妇科 [3]福建省妇幼保健院妇产科 [4]福建医科大学基础医学院生理学与病理生理学系

出　　处：《齐齐哈尔医学院学报》2012年第4期421-425,共5页Journal of Qiqihar Medical University

基　　金：福州市科技局项目(2007-S-124);福建省教育厅项目(JA04210;JA03104)

摘　　要：目的探讨宫颈鳞癌(SCC)局部肿瘤血管生成及其临床意义。方法应用免疫组化技术检测28例正常宫颈上皮(NCE),36例宫颈上皮内瘤变(CIN)和68例宫颈鳞癌组织局部微血管密度MVD(CD34标记)、细胞增殖指数PI(MIB-1标记)和微淋巴管密度LMVD(VEGFR-3标记)。结果 CD34标记的MVD在NCE、CIN及SCC组分别为(6.3±2.9)、(18.0±4.7)及(58.2±19.6)条。从NCE到CIN再到SCC,MVD显著升高(P<0.01)。在CIN中,MVD表达与PI显著正相关(r=0.373,P=0.025),而与LMVD无显著相关性(r=0.138,P=0.421)。在SCC中,MVD表达分别与PI及LMVD均无显著相关性(PI:r=-0.033,P=0.790;LMVD:r=0.114,P=0.354)。MVD在SCC组表达与间质浸润深度密切相关(P<0.01),而与患者年龄、FIGO分期、组织学分级、盆腔淋巴结转移、脉管浸润及生存率无关(P>0.05)。宫颈鳞癌突破深肌层间质浸润者MVD显著高于未突破深肌层间质浸润者(P<0.01)。结论 MVD表达上调可能促进宫颈异型上皮细胞增殖及宫颈鳞癌细胞浸润转移。Objective To investigate the tumor angiogenesis in squamous cell carcinoma of cervix and its clinical significance. Methods The microvessel density （MVD） labeled by CD34, proliferation index （PI） labeled by MIB-1 and lymphatic microvessel density （LMVD） labeled by VEGFR-3 protein in 28 cases of normal cervical epithelium （NCE）, 36 cases of cervical intraepithelial neoplasm （CIN） and 68 cases of squamous cell carcinoma of cervix （SCC） were detected by immunohistochemistry method. Results The MVD labeled by CD34 in NCE, CIN and SCC groups was 6. 3＋2. 9, 18. 0±4. 7 and 58.2＋19.6, respectively. The MVD increased remarkably from NCE to CIN and then to SCC accord- ingly （P （0.01）. The MVD in CIN group was positively correlated with PI （r=0. 373, P = 0. 025）, but not correlated with LMVD （r= 0.138, P = 0. 421）. The MVD in SCC group was both not correlated with PI and LMVD （PI.. r=--0. 033, P -- 0. 790; LMVD： r=0. 114, P = 0. 354）. In SCC group, the MVD positively correlated with depth of stroma involvement （P 〈 0.01）, but not correlated with pa- tients age, FIGO staging, histological grading, pelvic lymph node metastasis, intravascular infiltration and survival rate （P 〈 0.05）. In the cases with deeper stroma involvement, the MVD was significantly higher than those without the conditions mentioned above （P〈0.01）. Conclusions The over--expression of MVD may promote atypical cells proliferation of CIN and cancer cells invasion and metastasis of SCC.

关 键 词：宫颈肿瘤 微血管密度 增殖指数 微淋巴管密度 肿瘤转移 预后 免疫组织化学 

分 类 号：R737.31[医药卫生—肿瘤]