小檗碱对HL-60细胞增殖、凋亡及血管内皮生长因子受体2表达的影响  

Effect of Berberine on HL-60 Cell Proliferation,Apoptosis and Vascular Endothelial Growth Factor Receptor 2 Expression

作　　者：林少珠[1] 陈铭珍[1] 黄秀兰[1] 

机构地区：[1]广东医学院附属医院儿科,广东湛江524001

出　　处：《中国实验血液学杂志》2012年第2期262-267,共6页Journal of Experimental Hematology

摘　　要：本研究旨在观察小檗碱对人早幼粒白血病HL-60细胞的增殖、凋亡及血管内皮生长因子受体-2(VEGFR2)表达的影响。选用HL-60细胞体外培养,在不同浓度的小檗碱(6-96μg/ml)作用下,应用CCK-8法检测小檗碱对HL-60细胞的增殖抑制作用;应用流式细胞仪检测HL-60细胞的凋亡水平及细胞周期的分布;用RT-PCR及Western blot分别检测VEGFR2 mRNA及VEGFR2蛋白表达的变化。结果显示,小檗碱能抑制HL-60细胞的增殖,促进其凋亡,呈浓度和时间依赖关系。随着小檗碱浓度增加,G1期细胞增多,S期细胞减少,同时VEGFR2 mRNA和VEGFR2蛋白表达减少。结论:小檗碱可抑制HL-60细胞增殖,促进细胞凋亡,改变HL-60细胞周期,下调VEGFR2 mRNA及VEGFR2蛋白的表达。This study was aimed to investigate the effect of berberine on the proliferation and apoptosis of HL-60 cells,and the expression of vascular endothelial growth factor receptor 2（VEGFR2） in HL-60 cells. Berberine（6-96 μg/ml） was added to the HL-60 cell line culture medium,the CCK-8 method was used to reveal the inhibitory effect on cell proliferation,the flow cytometry was used to determine the apoptosis rate and cell cycle in HL-60 cells treated with berberine.The expression of VEGFR2 mRNA and protein were examined by RT-PCR and Western blot respectively.The results showed that the berberine inhibited the proliferation of HL-60 cells and induced their apoptosis in dose-and time-dependent manners.With the increased concentration of berberine,the percentage of HL-60 cells in G1 phase of cycle increased significantly,and the percantage of HL-60 cells in S phase decreased significantly.The expression of mRNA and protein of VEGFR2 decreased with the increased concentration of berberine.It is concluded that the berberine can inhibit HL-60 cell proliferation and induce HL-60 cell apoptosis.The expression of mRNA and proteins of VEGFR2 decreased after treatment with berberine.

关 键 词：小檗碱 HL-60细胞 血管内皮生长因子受体2 细胞周期 细胞凋亡 

分 类 号：R733.7[医药卫生—肿瘤] R979.1[医药卫生—临床医学]