雷公藤内酯醇对硼替佐米诱导多发性骨髓瘤细胞凋亡的影响  

Effects of Triptolide on Bortezomib-induced Apoptosis in Multiple Myeloma Cells

作　　者：杨洋[1] 董红娟[2] 高广勋[2] 王一苇[2] 顾宏涛[2] 陈协群[2] 

机构地区：[1]解放军总医院南楼血液科,北京100853 [2]第四军医大学西京医院血液科,陕西西安710032

出　　处：《中国实验血液学杂志》2012年第2期335-338,共4页Journal of Experimental Hematology

基　　金：国家自然科学基金资助项目;编号:30871089

摘　　要：本研究旨在探讨雷公藤内酯醇对硼替佐米诱导骨髓瘤细胞株NCI-H929(H929)凋亡的影响。通过MTT法检测不同浓度的雷公藤内酯和硼替佐米单用及联用对H929细胞的增殖抑制作用;采用Annexin Ⅴ-FITC/PI双染流式细胞术检测细胞凋亡率。结果表明:雷公藤内酯醇(10-100 ng/ml)和硼替佐米(10-100 nmol/L)单用和联用均可抑制H929细胞增殖,呈浓度依赖性,雷公藤内酯醇和硼替佐米联用组的细胞凋亡率明显高于单用硼替佐米组。经非抑制浓度的雷公藤内酯醇(10 ng/ml)联合硼替佐米(40 nmol/L)处理H929细胞24 h后的凋亡率明显高于单用硼替佐米组(P<0.05)。结论:雷公藤内酯醇能够显著增强硼替佐米对多发性骨髓瘤细胞的促凋亡活性。This study was purposed to investigate the effect of triptolide on bortezomib-induced apoptosis in multiple myeloma cell line NCI-H929（H929）.MTT assay was applied to detect the inhibitory effects of triptolide and bortezomib alone or combined at different concentrations on H929 cells,the cell apoptosis was assayed by flow cytometry with Annexin Ⅴ-FITC/PI staining.The results showed that both triptolide（10-100 ng/ml） and bortezomib（10-100 nmol/L） alone or combination inhibited the proliferation of MM cell line H929 in a concentration-dependent manner.The apoptotic rate of H929 cells in group of triptolide combined with bortezomib was much higher than that in groups of single drug or control;moreover,the apoptotic rate of H929 cells treated by non-inhibitory concentration of triptolide（10 ng/ml） combined with bortezomib（40 nmol/L） for 24 h was significantly higher than that by bortezomib alone（P0.05）.It is concluded that triptolide can significantly enhance the pro-apoptotic activity of bortezomib in MM cells.

关 键 词：雷公藤内酯醇 硼替佐米 骨髓瘤 NCI-H929细胞 

分 类 号：R733.3[医药卫生—肿瘤]