结直肠癌mP53和PCNA表达的相关性及临床意义  

Relationship between the Expression of Mutant P53 and PCNA and their Clinical Significances in Colorectal Cancer

作　　者：张艳霞[1] 邓亮[1] 杨秀兰[1] 刘春云[1] 郭敏芳[1] 

机构地区：[1]山西大同大学医学院,山西大同037009

出　　处：《现代生物医学进展》2012年第6期1101-1103,共3页Progress in Modern Biomedicine

摘　　要：目的:探讨结直肠癌中突变型P53基因(mP53)和增殖细胞核抗原(PCNA)表达的相关性及临床意义。方法:应用免疫组化二步法,检测60例结直肠癌组织及20例正常肠粘膜中mP53、PCNA的表达,结合临床病理资料进行统计分析。结果:60例结直肠癌中mP53阳性表达率65.0%,PCNA阳性表达率78.3%,20例正常肠粘膜中mP53、PCNA表达均为阴性(P<0.05)。mP53和PCNA阳性表达率在低分化组、浆膜层浸润组、淋巴结转移组均较高(P均<0.05)。mP53和PCNA表达呈正相关(r=0.58,P<0.05)。结论:mP53和PCNA在结直肠癌中表达均增高,二者与结直肠癌病理学分级、浸润深度和淋巴结转移有关,可作为判断结直肠癌预后的参考指标。Objective： To study the relationship between the expression of mutant P53（mp53）and proliferating cell nuclear antigen（PCNA）and their clinical significances in colorectal cancer.Methods： Immunohistochemistry was used to detect the expression of mu-tant p53 protein and PCNA in 60 tissues of colorectal cancer and 20 tissues of normal colorectal mucosa.The results were analysed with the clinical pathologic characters of colorectal cancer.Results： In the 60 tissues of colorectal cancer,the positive expression rate of mu-tant p53 and PCNA was 65.0% and 78.3%（P0.05）.In the 20 tissues of normal colorectal mucosa,the expressions of mutant P53 protein and PCNA were negative（P0.05）.In the group of lower differentiation,serosa invasion and lymph node metastasis of colorectal cancer,the positive expression rate of mutant p53 or PCNA was higher（P0.05）.The expression of mutant P53 has positive correlation with the expression of PCNA（r=0.58,P0.05）.Conclusion： The expression of mutant p53 or PCNA was correlated with differentiation,the depth of invasion and lymph node metastasis in colorectal cancer.therefore,they have prognostic value.

关 键 词：结直肠癌 突变型P53基因 增殖细胞核抗原 免疫组化 

分 类 号：R735.35[医药卫生—肿瘤]