去甲斑蝥素对H446细胞Id1mRNA表达的影响(英文)  

Norcantharidin Inhibits the Expression of Id1 mRNA in H446 Cells

作　　者：徐菲菲[1] 于小玲[1] 尚芳芳[2] 赵辉[2] 李红静[1] 

机构地区：[1]青岛大学医学院病理生理学教研室,山东青岛266021 [2]青岛中国人民解放军第401医院病理科

出　　处：《现代生物医学进展》2012年第7期1225-1228,共4页Progress in Modern Biomedicine

摘　　要：目的:观察去甲斑蝥素对小细胞肺癌H446细胞Id1mRNA表达的影响。方法:分别利用MTT法检测细胞生长活性;用划痕实验分析细胞迁移能力;采用Hoechst染色观察细胞凋亡;用实时荧光RT-PCR法测定H446中Id1mRNA的表达。结果:去甲斑蝥素对H446细胞的生长有明显的抑制作用,细胞的生长抑制率和凋亡率明显增加,细胞迁移距离明显缩短。去甲斑蝥素可抑制细胞内Id1mRNA的表达,其相对定量随去甲斑蝥素的浓度增大而减少。结论:在H446细胞中,去甲斑蝥素能抑制Id1mRNA的表达,这可能是去甲斑蝥素抑制细胞生长,迁移和诱导细胞凋亡的重要机制之一。Objective： To investigate the effects of the expression of Id1mRNA on human small cell lung cancer H446 cells.Methods： The cell viability of H446 was evaluated by MMT assay.The ability of cell migration was determined by scratch wound assay.The cell apoptosis was assayed by Hoechst staining.The quantity of Id1 mRNA of H446 cells was detected by real time quantitative reverse transcription-polymerase chain reaction（RT-PCR）.Results： The cell inhibition rate and cell apoptosis rate increased obviously by norcantharidin.The cell migrating distance was shorter in norcantharidin treated cell.Meanwhile,the relative quantity of Id1mRNA reduced significantly by norcantharidin.The quantity of Id1mRNA was inhibited by norcantharidin in a dose dependent manner.Conclusion： Norcantharidin can inhibit Id1mRNA in H446 cells,which may be one important mechanism of decreased cell viability,migration ability and induced cell apoptosis.

关 键 词：去甲斑蝥素 ID1 H446细胞 

分 类 号：R734.2[医药卫生—肿瘤]