bFGF诱导肺腺癌细胞系A549上皮间质转化  

bFGF Induced Epithelial-Mesenchymal Transition of Human Lung Adenocarcinoma Cell Line A549

作　　者：蔡宏凤 王慎旭 卢媛媛 王福[2] 张荣庆 曹丰 

机构地区：[1]第四军医大学第一附属医院西京医院心血管内科,陕西西安710032 [2]西安电子科技大学,陕西西安710071

出　　处：《现代生物医学进展》2012年第7期1241-1243,1264,共4页Progress in Modern Biomedicine

摘　　要：目的:探讨上皮间质转化(epithelial-mesenchymal transition,EMT)过程在肺癌侵袭转移中的作用。方法:体外培养A549细胞,以bFGF(10ng/ml)进行干预后,倒置相差显微镜观察细胞形态学变化;间接免疫荧光观察上皮细胞标志物E-cadherin和间质细胞标志物vimentin蛋白表达的变化;采用细胞划痕试验检测bFGF对A549细胞迁移能力的影响;采用transwell小室试验检测bFGF对A549细胞侵袭能力的影响。结果:bFGF(10ng/ml)干预后,在倒置相差显微镜下观察,A549细胞形态变成了梭形,形态如同成纤维细胞。间接免疫荧光显示A549细胞E-cadherin表达随时间延长逐渐减弱,而vimentin表达逐渐增强。细胞划痕试验显示,bFGF干预后细胞迁移能力提高。Transwell小室试验显示,bFGF干预后细胞侵袭能力提高。结论:bFGF在体外诱导肺腺癌细胞系A549细胞发生上皮间质转化,上皮间质转化是肺癌侵袭转移的重要机制之一。Objective： To investigate the effect of epithelial to mesenchymal transition（EMT） on the invasion and metastasis of lung cancer.Methods： A549 cells cultured in vitro were treated by bFGF（10ng/ml）,then the morphological changes were detected by inverted phase contrast microscopy;The expression of the epithelial marker E-cadherin and mesenchymal marker vimentin was detected by Indirect immunofluorescence;The effect of bFGF on migration of A549 was detected by scarification assay;The effect of bFGF on invasion of A549 was detected by transwell assay.Results： After treated by bFGF（10ng/ml）,A549 cells turn from pebble shape to fusiform shape,a myofibroblast-like morphology.The expression of E-cadherin decreased,and the expression of vimentin increased.Scarification test showed that the migration potential of A549 cells was significantly increased after bFGF treatment.The invasive capacity of A549 increased after bFGF treatment.Conclusion： bFGF may contribute to EMT in lung adenocarcinoma cell line A549.EMT is an important mechanism for invasion and metastasis of lung cancer.

关 键 词：碱性成纤维细胞生长因子bFGF A549细胞 上皮间质转化 肺癌 

分 类 号：R734.2[医药卫生—肿瘤]