ELISA法检测Zeta链联合血红蛋白电泳对地中海贫血筛查的价值  

Combined Zeta Peptide Detection by ELISA and Hemoglobin Electrophoresis in Thalassemia Screening

作　　者：尹晓林[1] 曾丽红[1] 周艳洁[2] 阮丽明[2] 周天红[1] 张天郎[1] 罗瑞贵[1] 李静[1] 张新华[1] 

机构地区：[1]解放军303医院血液科,广西南宁530021 [2]南宁市人口和计划生育服务中心

出　　处：《华南国防医学杂志》2012年第2期159-161,共3页Military Medical Journal of South China

基　　金：国家科技支撑计划项目(2006BAI05A00);国家973计划课题(2010CB530406)

摘　　要：目的探索适用于农村地中海贫血(thalassemia,地贫)的筛查模式。方法对南宁市郊农村750对育龄夫妇行血常规检测,平均红细胞体积(mean cell volume,MCV)下降者定义为地贫表型阳性。夫妇一方或双方地贫表型阳性者联合酶联免疫吸附剂测定(enzyme-linked immunosorbent assay,ELISA)法检测Zeta链和国产电泳仪行血红蛋白电泳,与地贫基因结果进行比对。结果 750对育龄夫妇中,261对(34.8%)夫妇任一方地贫表型阳性,接受Zeta链和血红蛋白电泳检测。97对夫妇一方HbA2增高者行β地贫基因检测,检出4对(0.41%)β地贫高危夫妇,即夫妻双方均携带β地贫基因,电泳结果与基因检测结果一致。α地贫基因检测结果表明,119例(22.7%)受试者携带--SEA。Zeta链诊断--SEA的漏诊率为5.9%(7例,其中5例为HbH病患者),误诊率为0.5%(2例)。以夫妇双方任一方Zeta阳性为标准,11对α地贫高危夫妇均可筛查出。结论ELISA法检测Zeta链联合血红蛋白电泳法是一种有效、经济、方便、易于在农村地区应用的地贫筛查模式。Objective To explore a suitable mehtod for thalassemia screening in rural area. Methods A total of 750 couples of childbearing age in the village of Nanning were studied.Cases with lower mean red cell volume（MCV） were termed as positive thalassemia phenotype.Couples with one or both as positive of thalassemia phenotype were further studied.Zeta peptide was detected by enzyme-linked immunosorbent assay（ELISA）.Hemoglobin electrophoresis was performed with domestic electrophoresis apparatus.Genotype detection of thalassemia was adopted as gold standard. Results A total of 261 couples were positive thalassemia phenotype.In 97 couples with increased HbA2 in one or both of the pairs,4 couples had high risk of β thalasemia and both of these couples had β thalasemia gene..Results of electrophoresis was in accordance with that of β thalasemia genotype detection.α thalassemia gene detection indicated 119 cases（22.7%） carried --SEA.As for zeta peptide detection for --SEA diagnosis,7 cases（5.9%） were missed（among whom 5 were patients with HbH disease）,and 2 cases were misdiagnosed（0.5%）.Under the diagnosis criteria that either of the couples had positive zeta peptide,11 couples with high risk of α thalassemia could be detected. Conclusion Combined Zeta peptide detection by ELISA and hemoglobin electrophoresis is effective,economic and convenient in screening thalassemia in rural areas.

关 键 词：地中海贫血 酶联免疫吸附剂测定 血红蛋白电泳 筛查 

分 类 号：R556.7[医药卫生—血液循环系统疾病]