高效液相色谱法测定保健食品中紫丁香甙  

Determination of syringin in health food by high performance liquid chromatography

作　　者：马莹[1] 王蕴馨[1] 张冠英[1] 边疆[1] 郭金芝[1] 

机构地区：[1]吉林省疾病预防控制中心理化检验科,吉林长春130062

出　　处：《中国卫生工程学》2012年第2期155-155,157,共2页Chinese Journal of Public Health Engineering

摘　　要：目的建立保健食品中紫丁香甙的高效液相色谱检测方法。方法样品经前处理后,过膜进样,利用高效液相色谱柱对不同的物质在流动相中的保留时间不同,以此作为定性依据,计算其含量。结果在1.5～50.0μg/ml浓度范围内相关系数为0.999 3,回收率92.5%～103.4%,RSD 5.21%～7.78%,最低检出量0.001 mg/100 g。结论本方法灵敏度高,检出限低,分离效果好,适用于保健食品中紫丁香甙的检测。Objective To establish high performance liquid chromatographic detection methods for detection of syringin in health foods.Methods Samples were selected by membrane after extraction.The difference in time of different samples preformed by high performance liquid chromatographic detection methods was regarded as a qualitative basis.Results The related coefficient in concentration was 1.5-50.0 μg/ml,r was 0.999 3.The recovery rate was 92.5%-103.4%,RSD was 5.21%-7.78%.The detection limit was 0.001 mg/100 g.Conclusion This method shows a high sensitivity,low detection limit and good separating effect.It can be used in detection of syringin in health foods.

关 键 词：高效液相色谱法 紫丁香甙 保健品 

分 类 号：R155.5[医药卫生—营养与食品卫生学]