CD28与B7.1共刺激PBLs受肝癌细胞诱导后的TCR Vβ基因表达  

Selective expression of TCR Vβ gene in PBLs costimulated by CD28+B7.1 and induced by hepatoma cell

作　　者：杨红[1] 李菁华[2] 黄树林[1] 

机构地区：[1]广东药学院分子生物学研究室,广东广州510224 [2]白求恩医科大学微生物学教研室,吉林长春130021

出　　处：《免疫学杂志》2000年第1期42-44,共3页Immunological Journal

基　　金：国家自然科学基金! ( 3 964 0 0 0 0 5 );广东省科教兴医"五个一"工程重点课题! (粤卫科 [1996]2 0号 )资助

摘　　要：目的研究T细胞受体(TCR)Vβ在外周血淋巴细胞(PBLs)中的表达。方法用单克隆抗体CD28+B7.1(CD80)共刺激正常人外周血淋巴细胞后作用于肝癌细胞(BEL-7402)。结果发现PBLsTCRVβ7基因在体外选择性扩增,表达水平为15%～25%,对照组为4%～8%。结论Vβ7基因对识别肿瘤抗原具有特异性,为肿瘤基因治疗和研究提供新的线索和实验依据。ObjectiveTo investigate selective expression of T cell receptor(TCR) Vβ gene in peripheral blood lymphocytes(PBLs).MethodsThe healthy human PBLs were costimulated by anti CD28 and anti CD80(B7.1) McAb and induced by hepatoma cells(BEL 7402).ResultsTCR Vβ7 gene were selectively amplified in PBLs induced by BEL 7402 cells and the expression level of Vβ7 was much higher in these cells than in controls (15%～25% vs 4%～8%).ConclusionVβ7 gene may specialize in recognizing tumor antigen. Our study offers new clues and experimental basis for gene treatment of tumor. [

关 键 词：肝肿瘤 T细胞受体 VΒ基因 PLBs 

分 类 号：R730.3[医药卫生—肿瘤]