AL-1与抗生素联用对绿脓杆菌胞外多糖和蛋白水解酶的影响  

The Effect of AL-1 in Combination with Antibiotics on Protease and Exopolysaccharide of Pseudomonas Aeruginosa

作　　者：王亮[1] 曾祥萍[1] 王玉强[1] 郑凯元[1] 傅和亮 张高小[1] 

机构地区：[1]暨南大学新药研究所,广东广州510632 [2]广东华南新药创制中心,广东广州510663

出　　处：《时珍国医国药》2012年第4期812-813,共2页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金-广东省联合基金重点项目(U1032007);国家自然科学基金面上项目(30772642)

摘　　要：目的探讨穿心莲内酯衍生物AL-1与阿奇霉素、红霉素、环丙沙星和磷霉素联合用药对绿脓杆菌胞外多糖和蛋白水解酶的影响。方法运用激光共聚焦显微镜观察各抗生素单用及和AL-1联用对PA胞外多糖的形成的影响;应用紫外可见分光光度计检测各抗生素单用及与AL-1联用对PA胞外蛋白水解酶含量的影响。结果 AL-1与抗生素联用可抑制PA胞外多糖形成及降低PA胞外蛋白水解酶含量,且效果强于AL-1与各抗生素的单独作用。结论 AL-1对各抗生素抑制PA胞外多糖的形成及减少胞外蛋白水解酶的含量具有协同作用。ObjectiveTo investigate the effect of AL-1 in combination with antibiotics on protease and exopolysaccharide of Pseudomonas aeruginosa（PA）. MethodsThe effects of AL-1 in combination with antibiotics on exopolysaccharide and protease were measured by laser scanning confocal microscope and UV-Vis spectrophotometer. ResultsThe production of both exopolysaccharide and protease were significantly reduced in the AL-1 combination with antibiotic group and the activities were shown more effective than using AL-1 or antibiotic group alone. ConclusionAL-1 has a synergistic effect with antibiotics acting on protease and exopolysaccharide of PA.

关 键 词：绿脓杆菌 生物膜 胞外多糖 蛋白水解酶 

分 类 号：R962[医药卫生—药理学]