融合酶的设计和应用研究进展  

Recent progress in fusion enzyme design and applications

作　　者：黄子亮[1] 张翀[1] 吴希[1] 苏楠[1] 邢新会[1] 

机构地区：[1]清华大学化学工程系,北京100084

出　　处：《生物工程学报》2012年第4期393-409,共17页Chinese Journal of Biotechnology

基　　金：国家自然科学基金(No.20836004);清华大学自主科研计划(No.2010Z02144)资助~~

摘　　要：酶的分子改造和重新设计是解决酶催化工业应用瓶颈的重要途径。基于融合蛋白设计的融合酶技术是分子酶工程的一个研究热点,已逐渐应用于多功能酶和酶靠近效应的构建与控制研究中,显示出重要的理论和应用研究价值。文中对近年来融合酶的分子设计策略和应用研究的进展进行了综述。首先介绍了融合酶的概念和特点,并对最近研究中出现的融合酶构建策略进行了归纳总结,重点阐述了不同种类连接肽对融合酶的影响及其可能机理。同时,对目前融合酶的应用研究进行了归纳和讨论。最后,结合本实验室的研究,指出了融合酶领域的关键问题并对其发展方向进行了探讨和展望。Engineering and redesign of enzymes are important to industrial biocatalysis. Fusion enzyme technology, based on fusion protein design, is frequently used in multifunctional enzyme construction and enzyme proximity control. Here, we reviewed the recent progress in molecular design strategy and application studies of fusion enzymes. The concept and features of fusion enzymes were introduced, followed by a systematical summary of the design strategy of fusion enzymes. In particular, the effects of different linker properties on fusion enzymes and their possible mechanisms were discussed. In addition, recent studies on fusion enzyme applications were also discussed. Finally, based on our own studicson fusion enzymes and the current research progress, the key problems in fusion enzyme technology and perspectives of this field were discussed.

关 键 词：酶工程 空间靠近效应 融合酶 融合蛋白 连接肽 

分 类 号：Q814[生物学—生物工程]