Dishevelled-1,3影响肺癌细胞侵袭机制的研究  

Mechanisms of Lung Cancer Cell Invasion Affected by Dishevelled-1 and 3

作　　者：赵月[1] 孙波[1] 高路[2] 韦强[3] 罗茹予[3] 殷舞[3] 王恩华[1] 

机构地区：[1]中国医科大学附属第一医院病理科,基础医学院病理学教研室,沈阳110001 [2]中国医科大学附属第一医院放疗科,沈阳110001 [3]广西壮族自治区人民医院病理科,南宁530021

出　　处：《中国医科大学学报》2012年第4期311-315,共5页Journal of China Medical University

基　　金：辽宁省教育厅高校科研计划(2009A768);广西自然科学基金资助项目(2010GXNSFB013078)

摘　　要：目的散乱蛋白(Dvl)亚家族成员在非小细胞肺癌中均存在过表达,但不同亚型对肺癌细胞侵袭能力的影响和可能的机制尚不清楚。方法检测转染Dvl-1和Dvl-3后肺癌细胞系T细胞转录因子(Tcf)转录活性的改变,β-连环蛋白(β-catenin)和p120连环蛋白(p120ctn)的表达,以及对细胞侵袭能力的影响。结果 Dvl-1和Dvl-3过表达后,肺癌细胞中p120ctn蛋白水平均上调,但Dvl-3可明显上调p120ctn mRNA水平。同时,Dvl-1过表达后Tcf转录活性明显上调,而Dvl-3未明显影响Tcf转录活性。应用β-catenin和p120ctn抗体抑制β-catenin和p120ctn蛋白作用后,可分别显著抑制由Dvl-1和Dvl-3增强的细胞侵袭能力。结论 Dvl-1可能主要通过经典Wnt通路中的β-catenin,而Dvl-3则可能主要通过与非经典Wnt通路相关的p120ctn影响肺癌细胞的生物学行为。Objective To explore the influence of Dishevelled-1 and 3 over-expression on the invasive ability of lung cancer cells and the underlying mechanisms.Methods The changes of β-catenin-dependent Tcf transcriptional activity,β-catenin and p120ctn expression were detected,together with the invasive ability of lung cancer cells after Dvl-1 and Dvl-3 transfection.Results Exogenous expression of Dvl-1 and Dvl-3 both up-regulated the protein level of p120ctn in lung cancer cells,while only Dvl-3 over-expression was accompanied by increasing p120ctn mRNA.However,Dvl-1 over-expression increased Tcf transcriptional activity significantly compared with Dvl-3.Meanwhile,invasion of Dvl-1 and Dvl-3-enhanced cells was inhibited by both β-catenin and p120ctn antibodies.Conclusion The biological behavior of lung cancer cells may be altered by Dvl-1 mainly through β-catenin which is an important molecule in canonical Wnt pathway,while Dvl-3 is mainly through p120ctn which is related to noncanonical Wnt pathway.

关 键 词：散乱蛋白 Β-连环蛋白 P120连环蛋白 肺癌 侵袭 

分 类 号：R743.2[医药卫生—神经病学与精神病学]