Effects of oral administration recombinant Saccharomyces cerevisiae expressing human-salmon calcitonin on hypercalcemia rats~  

作　　者：Jiang Yong Zhang Xuecheng Sun Pingnan Chen Yunsong 

机构地区：[1]College of M-arine Life Sciences,Ocean UniversitY of China,Qingda 0266003,P.R.Cllina [2]National Oceanographic Center,Qingdao 266071,P.R.China [3]Medical College of Shantou University,Shantou 515071,P.R.China

出　　处：《High Technology Letters》2012年第1期107-111,共5页高技术通讯（英文版）

摘　　要：Recombinant human-salmon calcxtonin （hsCT） was synthesized and expressea on the cell surtaces ot recombinant Saccharomyces cerevisiae （yAGA2-hsCT） using the pAGA2-hsCT expression vector. Expression of recombinant hsCT was verified by flow cytometer.The resorption activity of osteoclasts was inhibited by recombinant hsCT protein which released from the cell surface of yAGA2-hsCT. Blood calcium determination in rats showed the activity of yAGA2-hsCT following oral administration of 5 mg/kg lyophilized transformant yeast to hypercalcemia rats decreases the serum calcium from 2.82 ±0.023 mM to 2.662 ± 0.012 mM.

关 键 词：recombinant human-salmon calcitonin immunol fluorescence FACS OSTEOCLAST HYPERCALCEMIA saccharomyces cerevisiae 

分 类 号：Q523[生物学—生物化学] TQ464.7[化学工程—制药化工]