血清半乳糖凝集素3对胰腺癌的诊断价值  

Dignosis value of serum glypican-3 for pancreas cancer

作　　者：肖明兵[1] 谢玲[1] 倪温慨[1] 陈不尤[1] 陆翠华[1] 李小彦[1] 江枫[1] 倪润洲[1] 

机构地区：[1]南通大学附属医院消化内科,南通226001

出　　处：《中华胰腺病杂志》2012年第2期75-78,共4页Chinese Journal of Pancreatology

基　　金：江苏省“六大人才高峰”资助项目（2006073）;江苏省卫生厅资助项目（H200923）;南通市科技计划资助项目（S2010012）

摘　　要：目的运用时间分解免疫荧光（TRFIA）法检测血清半乳糖凝集素3（Galectin-3，Gal-3）水平，并探讨Gal-3对胰腺癌的诊断价值。方法采用固相双抗体夹心法建立检测血清Gal-3的TRFIA，探讨最佳实验条件。在最适条件下检测胰腺癌、胰腺良性占位、胰腺炎患者及健康对照者血清Gal-3水平，并联合检测血清CEA及CA19．9水平。结果TRFIA法检测血清Gal-3的线性为0—100μg／L，批内变异系数（CV）≤6．45％，批间CV≤8．68％，平均回收率为106．6％。胰腺癌患者血清Gal-3水平为4．93（0．85—23．80）μg／L，明显高于胰腺良性占位者的2．83（2．17—4．06）μg／L、胰腺炎患者的2．62（0．55—9．76）肌g／L和健康者的1．88（0．59—3．94）μg／L（P值均〈0．05）。以3．77μg／L为界，其诊断胰腺癌的敏感性为75．5％，特异性达90．9％。Gal-3与CEA及CAl9-9水平均无相关性（r=0．1321，P=0．3761；r=0．0920，P=0．5384），Gal-3联合CEA或CAl9-9检测，对胰腺癌的诊断敏感性可提高到92％。结论TRFIA法检测血清Gal-3具有较好的敏感性和稳定性；Gal-3有望成为新的胰腺癌标记物。Objective To establish the time-resolved fluoroimmunoassay （TRFIA） method for the detection of serum galectin-3 and investigate the clinical value of serum galectin-3 for the diagnosis of pancreas cancer. Methods Monoclonal anti-human galectin - 3 antibody and biotinylated polyclonal antibody were used to establish the sandwich TRFIA for detection of serum galectin-3. The optimal experimental condition was studied. Serum levels of galectin-3, CEA and CA19-9 in the patients with pancreatic cancer, benign pancreatic mass, pancreatitis, and healthy controls were measured. The diagnostic value of serum galectin-3, CEA and CA19-9 for pancreas cancer was studied. Results The linearity of the TRFIA for detection of serum galectin- 3 ranged between 0 to 100 μg/L. The within-run CV and between-run CV were ≤6.45% and ≤8.68%, respectively, and the average recovery was 106.6%. The level of serum galeetin-3 was 4.93 （0.85 ± 23.80 ） μg/L in pancreatic cancer group, which were significantly higher than those in benign pancreatic mass [ 2.83 （2.17 ± 4.06） μg/L ], panereatitis [ 2.62 （0.55 ± 9.76 ） μg,/L ], and healthy controls group [ 1.88 （0.59 ± 3.94） μg/L] （P 〈0.05）. By using 3.77 μg/L as the cut-off point, the sensitivity, specificity for the diagnosis of pancreatic cancer was 75.5% and 90.9%. The levels of Gal 3 and CEA, CA19-9 was not correlated （r = O. 1321, P = O. 3761 ; r = 0. 0920, P = 0. 5384）. Combined determination of galactin-3 and CEA, CA19-9 levels could increase the diagnostic sensitivity to 92%. Conclusions TRFIA method for the detection of galactin-3 is sensitive and stable. Galectin-3 could be a potentially novel serum tumor marker of pancreatic cancer.

关 键 词：胰腺肿瘤 半乳糖凝集素3 时间分解免疫荧光测定 血清 

分 类 号：R735.9[医药卫生—肿瘤]