神经中间丝蛋白NF-66与胰岛素瘤良恶性的关系  

Expression of neuronal intermediate filament protein （NF-66） and relationship with benign and malignancy of insulimomus

作　　者：刘贝[1] 肖雨[2] 朱丽明[1] 乔新伟[1] 向红丁[3] 陈杰[2] 陈原稼[1] 

机构地区：[1]中国医学科学院北京协和医学院北京协和医院消化内科,北京100730 [2]中国医学科学院北京协和医学院北京协和医院病理科,北京100730 [3]中国医学科学院北京协和医学院北京协和医院内分泌科卫生部内分泌重点实验室,北京100730

出　　处：《中华胰腺病杂志》2012年第2期89-91,共3页Chinese Journal of Pancreatology

基　　金：国家自然科学基金（30850002、8107205）;北京协和医院卫生部内分泌重点实验室专项基金

摘　　要：目的探讨神经中间丝蛋白NF-66能否作为判断胰岛素瘤良恶性的分子标志物。方法采用免疫组织化学染色法检测胰岛素瘤组织、瘤旁胰腺组织及3株胰岛素瘤细胞株NF-66蛋白的表达，应用单因素及多因素统计分析胰岛素瘤NF-66蛋白表达与肿瘤病理特征及患者预后的相关性。结果132例胰岛素瘤组织中102例（77％）表达NF-66蛋白，98例瘤旁胰腺组织均未见该蛋白表达（P=4．86×10^-3。）；3株胰岛素瘤细胞株均高表达NF-66蛋白。118例良性胰岛素瘤中96例（81％）表达NF-66，而14例恶性胰岛素瘤中6例（43％）表达NF-66，恶性胰岛素瘤的表达显著下降（P=0．003）。胰岛素瘤NF．-66表达与肿瘤大小（以3cm为界）、远处转移、远处并淋巴结转移均呈显著负相关（46％比81％，P=0．009；38％比81％，P=0．013；43％比81％，P=0．010）；而与患者性别、年龄、肿瘤复发和生存状态不相关。结论胰岛素瘤中NF-66表达下调与肿瘤的恶性生物学特征有关，可能成为潜在的鉴别胰岛素瘤良恶性的分子标志物。Objective To investigate whether neuronal intermediate filament protein （NF-66） could be used as a molecular marker for the determination of malignancy of insulinoma. Methods The expression of NF-66 protein was detected in insulinoma and para - cancerous tissues and 3 insulinoma cell lines by immunohistochemistry staining. The relationship between the expression of NF-66 protein and the clinicopathological characteristics, survival was analyzed by univariate and multivariate statistical analysis. Results Expression of NF-66 was found in 102（77% ） out of 132 insulinomas and none of 98 paired control （P = 4.86 × 10^-31 ）. NF-66 was highly expressed in 3 insulinoma cell lines. The expression of NF-66 was found in 96 （81%） out of 118 benign tumors （P =0.003）, while out of 14 malignant insulinomas, 6（43% ） were found to express NF-66 （P = 0.003 ）. The expression of NF-66 was significantly associated with tumor size （3 cm as the cut-off point）, distant metastasis （38% vs 81%, P =0.013） and distant plus lymph node metastasis （46% vs 81%, P =0.009）, respectively. The expression of NF-66 was not correlated with age, gender, recurrence and overall survival. Conclusions Down-regulation of NF-66 was significantly associated with tumor malignancy, suggesting that NF-66 could be a potentially novel molecular biomarker to distinguish malignancy from benign insulinoma.

关 键 词：胰岛素瘤 中间丝蛋白质类 诊断 鉴别 预后 

分 类 号：R736.7[医药卫生—肿瘤]