MMI-166对人胰腺癌细胞株SW1990体外增殖及凋亡的影响  

Effects of MMI-166 on proliferation and apoptosis in human pancreatic cancer SW1990 cell

作　　者：巩本刚[1] 徐怀勇[1] 成丕光[1] 高崇崇[1] 吴俊本[1] 

机构地区：[1]滨州医学院附属滨州市人民医院肝胆外科,滨州256600

出　　处：《中华胰腺病杂志》2012年第2期100-102,共3页Chinese Journal of Pancreatology

基　　金：山东省科技发展计划项目（2009GG20002096）

摘　　要：目的探讨基质金属蛋白酶抑制剂MMI-166对人胰腺癌SW1990细胞增殖和凋亡的影响。方法应用不同浓度（25、50、100μg／m1）的MMI-166处理人胰腺癌SW1990细胞24、48h。用四甲基偶氮唑蓝（MTT）法检测细胞增殖抑制率；采用AnnexinV—PI法检测细胞凋亡，流式细胞术检测细胞凋亡率。结果25、50、100μg/mlMMI-166处理细胞24h后，细胞生长抑制率分别为（34．23．4-3．87）％、（44．81±2．01）％、（53．91±1．74）％；48h的抑制率为（39．95±1．83）％、（52．26±3．46）％、（63．20±2．48）％，呈浓度及时问依赖性。24h的细胞凋亡率分别为（4．17．4-0．55）％、（8．22±0．70）％、（14．10±0．44）％；48h的细胞凋亡率为（11．19±0．47）％、（23．01±0．53）％、（28．10±0．52）％，均显著高于对照组的（0．09±0．12）％（P〈0．05）。结论MMI-166以浓度和时间依赖性抑制胰腺癌SW1990细胞增殖，诱导细胞凋亡。Objective To investigate the effects of MMI-166 on the proliferation and apoptosis of human pancreatic cancer SW1990 cells. Methods MMI-166 of different concentrations （25, 50, 100 μg/ml） were used to treat human pancreatic cancer SW1990 cell for 24, 48 h. Effect of MMI-166 on cell proliferation was detected by 3-（4,5-dimethyl-2-thiazole）-2-5-biphenly-tetrazole bromide （MTY） method and effect on cell apoptosis was tested by Annexin V-PI method and flow cytometry （FCM）. Results Twenty-four hours after MMI-166 treatment of different concentrations （25, 50, 100 μg/ml）, the inhibitory rates of the cells were （34.23 ±3.87）%, （44.81 ±2.01）%, （53.91± 1.74）%, and the corresponding values were （39.95 ± 1.83） % , （ 52.26 ± 3.46 ） % , （ 63.20 ± 2.48 ） % at 48 h, which suggested a time-and concentration- dependent manner. The cell＇ s apoptosis rates were （ 11.19 ± 0.47 ） % , （ 23.01 ± 0.53 ） % , （ 28.10 ± 0.52）% at 24 h, and the corresponding values were （11.19±0.47）%, （23.01 ±0.53）%, （28.10± 0. 52 ） % at 48 h, which were significantly higher than those in control group [ （0.09 ± 0. 12） %, P 〈 0. 05 ]. Conclusions MMI-166 can inhibit proliferation and induce apoptosis of human pancreatic SW1990 cell in a time- and concentration-dependent manner.

关 键 词：胰腺肿瘤 细胞系 肿瘤 金属蛋白酶类组织抑制剂 细胞增殖 细胞凋亡 

分 类 号：R735.9[医药卫生—肿瘤]