国产^(13)C-葡萄糖呼气试验检测糖尿病小鼠胰岛素抵抗的实验研究  

Assessment of insulin resistance in diabetic mice by ^(13)C-glucose breath test

作　　者：张敏[1] 管樑[1] 朱承谟[1] 郭睿[1] 卢伟京[2] 李良君[2] 杜晓宁[2] 李彪[1] 

机构地区：[1]上海交通大学医学院附属瑞金医院核医学科,上海200025 [2]上海化工研究院,上海200062

出　　处：《上海交通大学学报（医学版）》2012年第4期463-466,共4页Journal of Shanghai Jiao tong University:Medical Science

基　　金：上海市科委科研计划项目(08DZ2200203)~~

摘　　要：目的拟在糖尿病小鼠模型上建立一个无创的13C-葡萄糖呼气试验检测胰岛素抵抗(IR)的方法,并评估其与其他IR指数的相关性。方法四氧嘧啶尾静脉注射法制作糖尿病小鼠模型。多时间点采集小鼠13C-葡萄糖(5 mg/kg)灌胃后呼出的气样。红外线同位素能谱仪测定气样,以90 min 13CO2丰度变化值(C90 min)作为IR参数,评估糖尿病小鼠的IR情况及其在1 U外源性胰岛素干预后的IR变化。测定糖尿病小鼠的空腹血糖及空腹胰岛素值,并以此来计算临床IR指数:稳态模型胰岛素抵抗评价指数(HOMA-IR)和定量胰岛素敏感性检测指数(QUICKI)。分别分析C90 min与HOMA-IR或QUICKI之间的相关性。结果糖尿病小鼠的C90 min值明显低于正常对照小鼠,分别为(0.79±0.18)%和(1.19±0.31)%(t=2.470,P<0.05)。1 U外源性胰岛素干预后,糖尿病小鼠的C90 min值显著增加至(1.17±0.25)%(t=-2.522,P<0.05)。相关性分析显示,C90 min与HOMA-IR(r=-0.64,P<0.05)或QUICKI(r=0.88,P<0.01)均有良好的相关性。结论 13C-葡萄糖呼气试验能够灵敏反映糖尿病小鼠的IR变化,其检测结果与目前临床IR指数有良好的相关性。Objective To establish a noninvasive method of 13C-glucose breath test in assessment of insulin resistance（IR） based on the diabetic mice models,and explore its correlation with the other IR indexes.Methods Diabetic mice models were established by intravenous injection of alloxan tetrahydrate.For the 13C-glucose breath test,samples were collected at various time points after ingestion of 5 mg/kg 13C-labelled glucose.The value of 13CO2 at 90 min（C90 min） was calculated as the parameter of IR,and the effect of exogenous insulin（1 U） intervention on diabetic mice was evaluated.Fasting blood glucose and fasting insulin of diabetic mice were measured,and homeostatic model assessment of insulin resistance（HOMA-IR） and quantitative insulin sensitivity check index（QUICKI） were calculated.The correlation of C90 min with HOMA-IR or QUICKI was analysed.Results C90 min in diabetic mice was significantly lower than that in normal rats [（0.79±0.18）% vs（1.19±0.31）%,t=2.470,P0.05].C90 min in diabetic mice increased to（1.17± 0.25）% after exogenous insulin（1 U） intervention（t=-2.522,P0.05）.Correlation analysis revealed that C90 min was well related to HOMA-IR（r=-0.64,P0.05） or QUICKI（r=0.88,P0.01）.Conclusion 13C-glucose breath test may provide an effective noninvasive method for the assessment of IR in diabetic mice,which is well related to the IR indexes clinically available.

关 键 词：13C-葡萄糖 呼气试验 胰岛素抵抗 糖尿病小鼠 

分 类 号：R-332[医药卫生] R587.1