TLR2在胶质瘤细胞GL261的高表达及其激活后增强迁移的作用  

Triggering overexpressed TLR2 promotes migration of mouse glioma cells GL261

作　　者：王凡[1] 叶显宗[1] 杨浪[1] 崔有宏[1] 卞修武[1] 

机构地区：[1]第三军医大学西南医院病理学研究所西南癌症中心,重庆400038

出　　处：《成都医学院学报》2012年第1期28-31,36,共5页Journal of Chengdu Medical College

基　　金：国家重点基础研究发展计划(973计划)(No.2010CB529403);国家自然科学基金资助项目(No.30872297)

摘　　要：目的探讨小鼠胶质瘤细胞系GL261主要高表达的Toll样受体(TLRs)家族成员及其在GL261细胞迁移中的作用。方法运用实时荧光定量PCR检测TLRs在GL261细胞的表达情况;GL261细胞经TLR2配体Pam3CSK4刺激后,应用Transwell迁移实验检测其对迁移能力的影响;慢病毒稳定干扰TLR2表达后,观察Pam3CSK4刺激对GL261细胞迁移能力的改变。结果GL261细胞主要高表达TLRs家族中的TLR2;Pam3CSK4激活TLR2后显著增强GL261细胞迁移能力;沉默TLR2基因表达后,Pam3CSK4促进GL261细胞迁移能力的作用消失。结论小鼠GL261胶质瘤细胞TLRs家族中主要高表达TLR2,其激活具有促进GL261细胞迁移的作用,下调TLR2可抑制该细胞的迁移。Objective To explore the expression of Toll-like receptor family（TLRs）in mouse glioma cell line GL261 and effects of TLR2 activation on migration capacity of GL261 cells. Methods The expression of TLRs in GL261 was detected by qRT-PCR. Transwell migration assays were performed to determine the migration capacity of GL261 cells stimulated with Pam3CSK4, a TLR2 ligand. The change of migration capacity after silencing TLR2 in GL261 was examined by transwell assays. Results TLR2 of the TLRs family was highly expressed in GL261 cells. Pam3CSK4 stimulation enhanced migration capacity of GL261 cells was induced. Conclusion Triggering of TLR2 expressed by GL261 cell line enhanced its migration capacity, and the effect was reduced with down-regulation of TLR2.

关 键 词：Toll样受体2(TLR2) 胶质瘤细胞 侵袭 

分 类 号：R73-3[医药卫生—肿瘤]