多巴胺在聚亚甲基蓝/石墨烯修饰电极上的电化学行为研究  

Electrochemical Behavior of Dopamine at Poly(Methylene Blue)/Graphene Modified Glassy Carbon Electrode

作　　者：郭新美[1,2] 王宗花[1,2] 夏建飞[1,2] 张菲菲[1,2] 夏延致[1,2] 李延辉[1] 

机构地区：[1]青岛大学纤维新材料与现代纺织实验室国家重点实验室培育基地,山东青岛266071 [2]青岛大学化学化工与环境学院,山东青岛266071

出　　处：《分析测试学报》2012年第4期464-469,共6页Journal of Instrumental Analysis

基　　金：国家自然科学基金资助项目(20975056,81102411);山东省自然科学基金(ZR2011BZ004,ZR2011BQ005);NSFC-JSPS中日合作与交流项目(21111140014);生命分析化学国家重点实验室开放基金(SKLACLS1110);山东省泰山学者资助项目(TS20070711)

摘　　要：利用电聚合方法在石墨烯修饰的玻碳电极表面制备了聚亚甲基蓝/石墨烯修饰电极(PMB/GH/GCE)。采用循环伏安法(CV)和差分脉冲伏安法(DPV)研究了多巴胺(DA)和抗坏血酸(AA)在该修饰电极上的电化学行为。在pH 6.9的磷酸盐缓冲溶液中,DA和AA分别在0.208 V和-0.108 V处产生灵敏的氧化峰,与其在聚亚甲基蓝和石墨烯单层修饰电极上的电化学行为相比,两者的峰电流明显增加,峰电位差达316 mV。研究表明,电聚合方法使亚甲基蓝牢固地非共价修饰到石墨烯上,并产生协同增效作用,较好地提高了电极的灵敏度和分子识别性能,有利于在大量AA存在下实现对DA的选择性测定。在1.00×10-3mol/L AA的存在下,DA的差分脉冲伏安法峰电流与其浓度在1.00×10-7～5.00×10-3mol/L范围内呈良好的线性关系,检出限达1.00×10-8mol/L。将该方法用于盐酸多巴胺注射液的测定,结果满意。A poly（methylene blue）/graphene modified glassy carbon electrode（PMB/GH/GCE） was fabricated by electropolymerizing methylene blue on the surface of glassy carbon electrode modified with graphene.The electrochemical behaviors of dopamine（DA） and ascorbic acid（AA） at the prepared electrodes were investigated using cyclic voltammetry（CV） and differential pulse votammetric（DPV） methods.In pH 6.9 phosphate buffer solution（PBS）,the anodic peak potentials of DA and AA were 0.208 V and-0.108 V,respectively.In comparison with poly（methylene blue） modified glassy carbon electrode（PMB/GCE） and graphene modified glassy carbon electrode（GH/GCE）,the composite modified electrode（PMB/GH/GCE） had superior electrocatalytic activity toward the oxidation of DA and AA,with a peak to peak separation up to 316 mV.Studies showed that MB was firmly non-covalent modification of graphene by electropolymerizing method,and created synergistic effect which could improved sensitivity and molecular recognition ability of the modified electrodes and enabled the sensitive and selective detection of DA in the presence of large number of AA.A DPV technique for measurement for DA over the range of 1.00×10-7-5.00×10-3 mol/L was developed in the presence of 1.00×10-3 mol/L AA,with detection limit of 1.00×10-8 mol/L.The modified electrode was applied in the determination of dopamine hydrochloride injection solution with satisfactory result.

关 键 词：聚亚甲基蓝 石墨烯 修饰电极 多巴胺 抗坏血酸 

分 类 号：O657.1[理学—分析化学] O623.731[理学—化学]