核酸分子“光开关”作探针荧光光谱法检测转基因启动子病毒DNA特定序列  

Nucleic acid molecular “Light Switch” as the fluorescence probe to detect transgenic promoter-specific viral DNA sequences

作　　者：曾国平[1] 郑平[1] 

机构地区：[1]湖北第二师范学院化学与生命科学学院,武汉430205

出　　处：《华中师范大学学报（自然科学版）》2012年第2期174-179,共6页Journal of Central China Normal University：Natural Sciences

基　　金：湖北省教育厅科研项目(B20113001);湖北第二师范学院重点建设学科"应用化学"项目

摘　　要：合成核酸分子"光开关"Ru(phen2)(dppx)2+,并以其作为荧光探针检测转基因花椰菜花叶病毒35S启动子(CaMV 35S)序列片段(5’-CATCGTT GAAGA TGCCTCTGCC G-3’).通过对检测条件的优化,测定CaMV 35S病毒DNA的线性范围为1.4×10-9～6.9×10-8 mol.L-1,线性关系:ΔI=1.6913C+5.5783(式中ΔI为标准溶液的荧光强度与空白溶液的荧光强度之差),R2=0.9938,检出限为8.6×10-10 mol/L.实验表明该方法有操作简单、灵敏度高和选择性好等特点,同时可以较好地识别碱基错配序列和非互补序列ssDNA.Nucleic acid molecule ＂Light Switch＂ Ru（phen）2 （dppx）^2＋ was synthesized , and used as a fluorescent probe to detect the genetically modified cauliflower mosaic virus 35S promoter （CaMV 35S） sequence fragment （5＇-CATCGTT GAAGA TGCCTCT- GCC G-3 ＇）. Through the optimization of detection conditions, the linear range of CaMV 35S viral DNA was 1.4 × 10^-9- 6.9 × 10^-8 mol / L, and the calibration curve was △I=1. 6913C＋5. 5783, R^2 =0. 9938, and the detection limit was 8.6×10^-10 mol / L. The results showed that the method is simple, sensitive and selective, and displays great potential for the determination of base mismatch sequence and non-complementary sequence of ssDNA.

关 键 词："光开关"Ru(phen)2(dppx)2+ 花椰菜花叶病毒 DNA片段 定量检测 

分 类 号：O657[理学—分析化学]