食管鳞癌细胞周期调控基因CyclinD1蛋白和增殖细胞核抗原的表达  

Expression of CyclinD1 and proliferating cell nuclear antigen in esophageal carcinoma

作　　者：卢宝安[1] 韩英杰[2] 任秀宝 

机构地区：[1]天津市静海县医院普外科,天津静海301600 [2]天津医科大学病理科,天津303001 [3]天津肿瘤医院放免科,天津300060

出　　处：《河北医学》2000年第1期10-14,共5页Hebei Medicine

摘　　要：目的：探讨ＣｙｃｌｉｎＤ１ 和增殖细胞核抗原（ＰＣＮＡ）在食管鳞癌，癌旁正常上皮和非典型增生上皮的表达特点，并分析了ＣｙｃｌｉｎＤ１ 在食管鳞癌发生，发展中的作用及与临床病理特点的关系。方法：应用免疫组织化学Ｓ—Ｐ法对８２ 例食管鳞癌，３３ 例非典型增生上皮和２３ 例正常粘膜上皮进行了ＣｙｃｌｉｎＤ１ 和ＰＣＮＡ检测。结果：８２ 例食管鳞癌中５７ 例阳性表达，阳性率为６９．５％ ，非典型增生组阳性率为３６．４％ （１２／３３），正常粘膜上皮阳性率为８．７％ （２／２３），ＰＣＮＡ的平均阳性率在三组分别为７５．６％ ±１３．５％ ，３６．６％ ±９．８％ ，４．５％ ±０．５％ ，食管鳞癌中ＣｙｃｌｉｎＤ１ 和ＰＣＮＡ 表达有显著的正相关（Ｐ＜ ０．０５），ＣｙｃｌｉｎＤ１ 和ＰＣＮＡ的表达与组织学分级有关（Ｐ＜ ０．０５），而与其它临床病理特征无关。结论：①食管癌组ＣｙｃｌｉｎＤ１ 阳性率显著高于非癌组，且非典型增生组亦明显高于正常组（Ｐ＜ ０．０５），提示ＣｙｃｌｉｎＤ１ 在食管鳞癌的发生，发展中起重要作用。②ＣｙｃｌｉｎＤ１ 可作为提示食管鳞癌预后的参考指标之一。Objective:To study the expression of CyclinD1 and proliferating cell nuclear antigen(PCNA)in esophageal carcinoma and its correlation with clinic pathologic features.Method:Immunohistoche mical assay was performed with a S P kit to detect the expression of Cyclind1 and PCNA in 82 cases of esophageal carcinoma,23 cases of normal epithelium and 33 cases of atypical hyperplasia. Results:①57 cases of esophageal carcinoma ,12 cases of atypical hyperplasia and 2 cases of normal epithelium express CyclinD1,their positive rates are 69 5%,36 4%and 8 7% respectively ②all cases of esophageal carcinoma express PCNA,its mean positive rate is 67 4%③The statistically significant association is found between the expression of CyclinD1& PCNA and histological grade ④the statistically significant association is found between the expression of CyclinD1 and the expression of PCNA Conclusion:①CyclinD1 may play an important role in esophageal carcinoma genesis ②Combination of CyclinD1 and PCNA expression seems a reliable parameter for judging malignant degree of esophageal carcinoma.

关 键 词：食管鳞癌 CYCLIND1 PCNA 细胞周期 

分 类 号：R730.3[医药卫生—肿瘤]