神经导向因子-1在急性肾损伤模型大鼠尿液中的含量变化  

Changes in Urine Netrin-1 and Its Application in Rat Model of Acute Kidney Injury

作　　者：陈玉[1] 蔡利励[1] 郑剑锋[1] 王小中[1] 

机构地区：[1]南昌大学第二附属医院检验科,南昌330006

出　　处：《南昌大学学报（医学版）》2012年第2期11-13,22,共4页Journal of Nanchang University：Medical Sciences

基　　金：江西省卫生厅课题(20111046)

摘　　要：目的探讨神经导向因子-1(Netrin-1)作为急性肾损伤(AKI)早期标志物的价值。方法将40只SD大鼠按随机数字表法分为缺血再灌注组、叶酸处理组、脂多糖处理组和顺铂处理组,分别建立缺血再灌注、顺铂、叶酸和脂多糖诱导的大鼠AKI模型,每组10只。记录4组大鼠1、3、6、24、48、72h尿Netrin-1水平及血肌酐(Scr)浓度的变化。结果肾缺血再灌注组:灌注3h后尿Netrin-1显著上升(为基础值的20倍,P<0.01),在6h达峰值,然后慢慢下降,48h后接近正常水平,而Scr在再灌注后24h才显著增加达峰值。顺铂、叶酸、脂多糖诱导的3组大鼠:尿Netrin-1水平均在注射后1h增加,分别在损伤后的6、3、6h达最高峰,而Scr分别在24、6、6h后才显著增加(均P<0.01)。结论尿Netrin-1是一种有前景的肾损伤早期标志物。Objective To investigate the value of Netrin-1 for the early detection of acute kidney injury（AKI）.Methods The AKI was induced in 40 SD rats by ischemia-reperfusion（ischemia-reperfusion group,n=10）,folic acid（folic acid,n=10）,lipopolysaccharide（LPS group,n=10） and cisplatin（cisplatin group,n=10）,respectively.The levels of urine Netrin-1 and serum creatinine were measured 1,3,6,24,48 and 72 hours after treatment.Results The levels of urine Netrin-1 significantly increased 20-fold 3 hours after renal ischemia-reperfusion relative to baseline（P0.01）,reached the peak after 6 hours,and then slowly decreased to normal levels after 48 hours.However,serum creatinine concentrations significantly increased up to peak 24 hours after reperfusion.In rats treated with cisplatin,folic acid or LPS,urine Netrin-1 levels increased 1 hour after induction,peaked 6,3,6 hours after injury.But,serum creatinine concentrations significantly increased after 24,6,6 hours（all P0.01）.Conclusion Urinary Netrin-1 is a promising biomarker for early detection of renal injury.

关 键 词：急性肾损伤 血肌酐 神经导向因子-1 动物 实验 大鼠 

分 类 号：R-332[医药卫生]