丹皮酚对大肠癌细胞端瞄聚合酶的影响  

The effect of Tankyrase in colorectal cancer cells by paeonol

作　　者：夏士涛[1] 谭诗云[1] 朱卫芳[1] 刘长青[2] 

机构地区：[1]武汉大学人民医院消化内科,湖北武汉430060 [2]荆门市第一人民医院消化内科

出　　处：《胃肠病学和肝病学杂志》2012年第4期363-365,共3页Chinese Journal of Gastroenterology and Hepatology

基　　金：武汉市科技局重点项目(201060938363-05);湖北省科技厅自然科学基金(2009CDB097)

摘　　要：目的探讨丹皮酚对大肠癌细胞端瞄聚合酶的影响。方法应用RT-PCR分别检测实验组及对照组Tankyrase1 mRNA的表达率。结果 RT-PCR扩增的Tankyrase1 cDNA产物(目的基因与内参拷贝数的比值)在大肠癌细胞中的表达(0.875±0.037)较正常对照组升高(0.1532±0.108)(P<0.05),而在给予丹皮酚干预后的Tankyrase1(0.356±0.018)表达下降(P<0.05),但在正常大肠细胞组(0.1532±0.108)与正常细胞加丹皮酚组中(0.2379±0.012)无显著性差异(P>0.05)。结论丹皮酚在15.63～250 mg/L浓度范围内影响HT-29细胞中Tankyrase1 mRNA的表达。Objective To investigate the effect of paeonol on Tankyrase in coloretal cancer cell.Methods The expression rate of Tankyrase1 was detected in the experimental group and control group by RT-PCR.Results The expression of Tankyrase1 cDNA in experimental group was highter than that in the control group,but the expression of Tankyrase1 cDNA was lower by paeonol after the interrection,there was no significant difference in the intestinal cell group and the postintervented group of paeonol.Conclusion The paeonol at the concentration of 7.81~250 mg/L could influence the expression of Tankyrase1 mRNA in HT-29 cell.

关 键 词：丹皮酚 大肠癌 端瞄聚合酶 

分 类 号：R735.3[医药卫生—肿瘤]