大豆子叶节丛生芽的诱导研究  

Induction of Multiple Shoots from Soybean Cotyledonary Node

作　　者：孙昕[1] 闫帆[1] 赵健如[1] 陈虹地[1] 翟莹[1] 王英[1] 李景文[1] 王庆钰[1] 

机构地区：[1]吉林大学植物科学学院,吉林长春130062

出　　处：《大豆科学》2012年第2期184-187,192,共5页Soybean Science

基　　金：转基因生物新品种培育重大专项子课题(2011ZX08004-003);国家自然科学基金面上项目(30971808);农村科技成果转化基金项目(2010GB23600676);"十二五"农村领域国家科技计划子任务(2011BAD35B06-2)

摘　　要：选用吉大豆1号、吉大豆2号、吉林47和东农42共4个基因型的大豆子叶节作为外植体进行离体再生,筛选出丛生芽诱导率较高的大豆基因型吉林47。在此基础上,以吉林47的大豆子叶节为外植体,研究了外植体消毒时间、发芽时间和条件对大豆子叶节再生的影响,并设计正交试验对芽诱导及伸长阶段的激素配比进行了研究。得出吉林47较适合氯气灭菌时间为6～10 h,最适萌发条件为16 h光+8 h暗光周期条件下培养5～7 d。最终确定芽诱导阶段添加6-BA浓度为2 mg.L-1、IBA浓度为0.1 mg.L-1;14 d后继代伸长阶段添加IAA浓度0.3 mg.L-1、GA浓度为0.5 mg.L-1。In order to establish a cotyledonary node regeneration system for soybean（Glycine max L.）,the cotyledonary node of 4 soybean genotypes were chosen as explants and Jilin 47,which had the maximum induction rate of multiple shoots,was screened out.Then Jilin 47 was used as explants to study the effect of sterilization hours,germination condition,concentration of auxin on induction rate,elongation rate and average shoot.When sterilized for 6-10 h and germinated 5-7 days in 16 h/8 h photoperiod,Jilin 47＇s germination,shoot induction and elongation rate were higher than other treatments.The best hormone combination for multiple shoots induction were 2 mg·L-16-BA and 0.1 mg·L-1 IBA,and that for shoots elongation were 0.3 mg·L-1 IAA and 0.5 mg·L-1 GA.

关 键 词：大豆子叶节 再生 丛生芽 吉林47 

分 类 号：S565.1[农业科学—作物学]