渐进性咬合紊乱对大鼠髁突软骨中IL-1β、IL-6和TNF-α表达的影响  

Effect of experimentally created disordered occlusion on the expression of IL-1β,IL-6 and TNF-α in condylar cartilage of rats

作　　者：代康[1] 焦凯[1] 于世宾[1] 刘晓东[1] 王美青[1] 

机构地区：[1]第四军医大学口腔医学院解剖生理学教研室,陕西西安710032

出　　处：《上海口腔医学》2012年第2期121-126,共6页Shanghai Journal of Stomatology

基　　金：国家自然科学基金(30928028)~~

摘　　要：目的:探讨渐进性咬合紊乱致大鼠髁突软骨异常改建过程中IL-1β、IL-6和TNF-α的表达变化情况。方法:32只8周龄SD雌性大鼠,在实验组大鼠第一、第二和第二、第三磨牙之间填塞正畸用橡皮筋,推第一、第三磨牙移动约0.8mm,分别于8周和12周后取双侧颞下颌关节,苏木精—伊红染色观察组织形态学变化,免疫组化及RT-PCR方法检测IL-1β、IL-6和TNF-α的表达情况,采用SPSS12.0软件包对数据进行统计学分析。结果:8周(4/8)和12周(6/8)实验组髁突软骨出现典型退行性变,IL-1β的表达在各组间无显著差异(P>0.05),8周实验组IL-6(P=0.009)和TNF-α(P=0.001)的表达显著高于8周对照组;12周实验组TNF-α的表达显著低于8周实验组(P=0.004),与同龄对照组相比无显著差异。结论:实验早期,IL-6和TNF-α参与了实验性咬合紊乱所致髁突软骨异常改建活动,但在观察期内,IL-1β无明显参与的证据。PURPOSE： To investigate the effect of the experimentally created malocclusion（ECDO） on the expression of IL-1β,IL-6 and TNF-α in mandibular condylar cartilage of Sprague Dawley（SD） rats.METHODS： In the experimental group,the malocclusion was created by moving the first and third molars 0.8 mm away.HE and immunohistochemical staining were performed on the mandibular condyle at the end of the 8th or 12th week.Gene expression was analyzed by real-time PCR.The data was processed using SPSS12.0 software package.RESULTS： Degenerative changes were found in the experimental group,the level of IL-1β was not significantly different among the groups（P〉0.05）,while the expression of IL-6 in 8-week ECDO subgroup was significantly higher than that in 8-week control subgroup（P=0.009）,and the expression of TNF-α in 8-week ECDO subgroup was significantly higher than that in 8-week control subgroup（P=0.001） and 12-week ECDO subgroup（P=0.004）.CONCLUSIONS： At the early period,IL-6 and TNF-α,but not IL-1β,play an important role in the degenerative remodeling procedure in rat condylar cartilage induced by ECDO.

关 键 词：咬合 关节 软骨 IL-1Β IL-6 TNF-Α 

分 类 号：R782.6[医药卫生—口腔医学]