温石棉与烟溶液作用后人肺泡上皮细胞DNA的损伤及修复  

Effect of chrysotile and cigarette smoking solution on DNA damage and repair in human alveolar epithelial cells

作　　者：王起恩[1] 柳德米拉.曲路 韩春华[3] 顾志刚[2] 刘世杰[1] 

机构地区：[1]北京医科大学公共卫生学院劳动卫生教研室,100083 [2]北京医科大学公共卫生学院预防医学系,100083 [3]首都医科大学劳动卫生教研室

出　　处：《中华预防医学杂志》2000年第1期25-27,共3页Chinese Journal of Preventive Medicine

摘　　要：目的　了解温石棉与烟溶液单独及联合作用对细胞DNA损伤后的修复影响。方法将人肺泡上皮细胞用温石棉或烟溶液作用 1h ,即刻去毒培养不同时间后 ,用单细胞凝胶电泳技术测定DNA链断裂情况 ,从而分析DNA的修复功能。结果　 4 0 μg ml温石棉与 2 5× 10 - 4 支 ml烟溶液作用 1h后 ,均可明显引起DNA链断裂 ,温石棉染毒组在继续培养 3h后 ,DNA可出现明显修复 ,4h的修复率达 3 0 6% ;烟溶液染毒组在继续培养 2h后即可出现明显修复 ,4h的修复率达 65 2 % ;二者联合作用后的修复情况与烟溶液单独作用相似。结论　石棉所致的DNA损伤比烟溶液所致的损伤更难修复。Objective Tostudytheeffectofchrysotileandsolutionofcigarettesmokeseparatelyor combinedonDNAdamageandrepair.Methods Single cellsgelelectrophoresisassaywasusedtodetectbreakin DNAstrandandtoanalyzeitsrepairfunctioninhumanalveolarepithelialcelllineA5 4 9,whichwereexposedto chrysotileorcigarettesmokesolutionseparatelyorcombinedforonehourandthenimmediatelyculturedfor differenttime .Results BreakinDNAstrandswasinducedobviouslyinA5 4 9cellsafterexposureto 4 0 μg mlof chrysotileor2 5× 10 - 4 cig mlofcigarettessmokesolutionforonehour.RepairforDNAbreakcouldbeseen clearlyinchrysotile treatedcellsaftercontinuingcultureforthreehours,and 3 0 6%hadbeenrepairedafter cultureforfourhours.Repaircouldalsobeseenclearlyincigarettesmokesolution treatedcellsaftercontinuing culturefortwohours,and 65 2 %hadbeenrepairedaftercultureforfourhours.RepairofDNAincellsexposedto bothchrysotileandcigarettesmokesolutioncombinedwassimilartothatexposedtocigarettesmokesolutiononly. Conclusion DamagetoDNAcausedbychrysotileismoredifficulttorepairthanthatinducedbycigarettesmoking solution .

关 键 词：石棉类 蛇纹石 吸烟 DNA损伤 DNA修复 

分 类 号：R73-35[医药卫生—肿瘤]