糖基化终产物对人牙周膜细胞增殖和NF-κB mRNA表达的影响  

The influence of advanced glycation end products on the proliferation and NF-κB expression of human periodontal ligament fibroblasts

作　　者：房明[1] 汪涛[1] 王欣[1] 王新文[1] 王勤涛[1] 

机构地区：[1]第四军医大学口腔医学院,陕西西安710032

出　　处：《牙体牙髓牙周病学杂志》2012年第4期200-202,226,共4页Chinese Journal of Conservative Dentistry

基　　金：十一五国家科技支撑计划课题(2007BAI18B02)

摘　　要：目的:研究比较在体外高糖、糖基化终产物(advanced glycation end of products,AGEs)刺激环境下对人牙周膜细胞(hPDLFs)增殖能力和NF-κB mRNA表达的影响。方法:选取因正畸治疗而拔除的前磨牙体外分离培养人牙周膜细胞,根据不同的刺激环境分为阴性对照组(5.5 mmol/L葡萄糖)、高糖组(25 mmol/L葡萄糖)、AGEs组(100μg/mL AGEs)3组。培养12、24、36 h,3个时间点,使用Real Time PCR检测各组NF-κBmRNA的表达差异情况;流式细胞仪检测培养hPDLFs 36 h时细胞增殖差异情况。结果:在培养12、24、36 h,高糖组和AGEs组NF-κB mRNA的表达均高于阴性对照组(P<0.05),其中AGEs组最高,与高糖组相比,24、36 h时均有统计学差异(P<0.05);培养36 h AGEs组hPDLFs增殖能力明显低于阴性对照组和高糖组(P<0.05);高糖组与阴性对照组相比无统计学差异(P>0.05)。结论:AGEs能够显著的抑制hPDLFs增殖,并上调NF-κB的表达呈时间依赖性。AIM： To study the influence of high glucose environment and advanced glycation end products（AGEs） on the proliferation and NF-κB mRNA expression of human periodontal ligament fibroblasts（hPDLFs） in vitro.METHODS： The hPDLFs were isolated from premolars extracted for orthodontic purpose.Cells were divided into 3 groups： negative control group（5.5mmol/L glucose）,high glucose group（25mmol/L glucose） and AGEs group（100μg/mL AGEs）.After 12,24 and 36 h of culture,real time PCR was used to assess the NF-κB mRNA level.After 36h of culture,flow cytometry was applied to test the proliferation of 3 groups.RESULTS： At 12 h,24 h and 36 h,the NF-κB mRNA expression was elevated in AGEs group and high glucose group（P〈0.05）.AGEs group exhibited higher NF-κb mRNA expression than high glucose group at 24 h and 36 h（P〈0.05）.After 36 h of culture,the proliferation of hPDLFs in AGEs group was inhibited compared to the other two groups（P〈0.05）.There was no statistical significance between high glucose group and negative control group（P〈0.05）.CONCLUSION： AGEs can strongly inhibit the proliferation of hPDLFs and enhance their NF-κB expression in a time dependent manner.

关 键 词：糖基化终产物 糖尿病 牙周炎 NF-ΚB 

分 类 号：R780.1[医药卫生—口腔医学]