HPLC测定头花蓼及制剂热淋清颗粒中没食子酸的含量  

Determ Ina Tion of Gallic Acid in Polygonum Capita Tum and Relinqing Granules by HPLC

作　　者：王爽[1] 张丽艳[2] 谢宇 潘雯婷[2] 李孟林 

机构地区：[1]浙江中医药大学药学院,杭州310053 [2]贵阳中医学院药学系,贵阳550002 [3]贵州威门药业股份有限公司,贵阳550000

出　　处：《中国实验方剂学杂志》2012年第9期112-115,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：黔科合重大专项(20086020);十一五科技支撑项目(2009BAI74B01-4);[2010]筑科农合同字第1-中~12号

摘　　要：目的:建立头花蓼及热淋清颗粒中没食子酸的含量测定方法。方法:采用反相高效液相色谱法,DiamonsilTM(钻石)C18柱(4.6 mm×250 mm,5μm),流动相甲醇-水-N,N-二甲基甲酰胺-冰醋酸(1∶95∶3∶1),流速1.0 mL·min-1,检测波长272 nm,柱温25℃。结果:没食子酸在0.139 6～1.396 0μg线性良好(r=0.999 97)。头花蓼平均回收率99.35%,RSD为2.61%,热淋清颗粒平均回收率97.86%,RSD为0.73%。结论:该法重复性好,专属性强,可用于控制头花蓼药材及其单方制剂热淋清颗粒的质量。为头花蓼药材规模化种植提供科学依据。Objective： To establish an HPLC method for determination of the contents of gallic acid in Polygonum capitatum and Relinqing granules.Method： The reserved HPLC method was achieved.Diamonsil C18 colunm（4.6 mm×250 mm,5 μm） with MeOH-H2O-DMF-HAc（1∶ 95∶ 3∶ 1） as amobile phase at flow rate of 1.0 mL·min-1and detection wavelength was at 272 nm and the column temperature was at 25 ℃.Result： The calibration curve was linear in the range of 0.139 6-1.396 0 μg（r=0.999 97）.The average recovery rate of gallic acid in P.capitatum was 99.35%（RSD 2.61%）,and was 99.35%（RSD 0.73%） in Relinqing granules.Conclusion： The method is reliable,specific and with good repeatability.It can be used for the quality control of P.capitatum and Relinqing granules and provide a science bases for the planting of polygonum cap itatum.

关 键 词：头花蓼 热淋清颗粒 没食子酸 

分 类 号：R284.1[医药卫生—中药学]