苯并[a]芘对马氏珠母贝D型面盘幼虫发育的影响  

Impacts of B[a]P on the Development of Pinctada martensii D-shaped Veliger Larvae

作　　者：杨宝[1] 刁晓平[1] 谢嘉[1] 赵春风[1] 周海龙[1] 

机构地区：[1]海南大学农学院、海南省热带生物资源可持续利用重点实验室,海口570228

出　　处：《生态毒理学报》2012年第2期215-219,共5页Asian Journal of Ecotoxicology

基　　金：国家自然科学基金(31160126;41161077);海口市重点科技项目(2009-47);海南省自然科学基金项目(309010);海南大学重点科技项目(hd09xm51);海南大学211工程建设项目(QNJS-2011-13);海南大学博士启动基金(kyqd1102)

摘　　要：苯并芘(B[a]P)已广泛分布于海洋环境中,低等海洋无脊椎动物从受精卵开始整个生长发育过程都面临着B[a]P等多环芳烃类污染物(PAHs)的威胁。本实验以热带海洋优势贝类马氏珠母贝(Pinctada maetensii)作为材料,研究B[a]P对马氏珠母贝D型面盘幼虫发育的影响。将幼虫暴露于不同浓度(1、2、4、10和15μg·L-1)B[a]P中,在暴露后第12、24、36、48、72、84小时,分别测定其D型面盘幼虫的死亡率和畸形数。结果表明:B[a]P对D型面盘幼虫死亡率的影响明显,死亡率与时间呈正相关,其中4和10μg·L-1浓度组的响应最敏感。在1～10μg·L-1浓度范围内,死亡率与浓度呈正相关;B[a]P暴露对马氏珠母贝D型面盘幼虫的形态也有显著影响,且随着浓度的增大产生畸形个体的时间越短。另外,B[a]P对马氏珠母贝D型面盘幼虫的半数致死浓度(LC50)随着时间的推移逐渐降低后趋于稳定,48h时LC50趋于稳定,为21.56μg·L-1。研究表明,B[a]P对马氏珠母贝D形幼虫的发育具有不利影响,并可能影响其种群结构。B[a]P is the most studied polycyclic aromatic hydrocarbons （PAHs）,which widely exists in the marine environment.Invertebrates are threatened by PAHs in their whole life.In this paper,the impacts of B[a]P on the development of D-shaped veliger larvae of Pinctada martensii were studied.When fertilized eggs growed up to D-shaped veliger larvae,they were exposed to B[a]P at six different concentrations of 0,1,2,4,10 and 15 μg·L-1.The mortality and aberration rate were calculated after D-shaped veliger larvae exposed for 12,24,36,48,72,and 84 hours.Results showed that the mortality was positive to exposure time.B[a]P has a significantly toxic effect on D-shaped veliger larvae of Pinctada martensii.Larvae in 4 and 10 μg·L-1 groups were much more sensitive than that in other groups.The morality was positive to the concentration of B[a]P in the range of 1～10 μg·L-1.B[a]P could significantly deform D-shaped veliger larvae.Moreover,exposed to high concentration of B[a]P resulted in the early appearence of the abnormal individual.Additionally,LC50 gradually decreased then kept a constant.48h-LC50 was 21.56 μg·L-1.The results indicated that B[a]P had negative effects on the developement of D-shaped veliger larvae of Pinctada martensii and their populative structure.

关 键 词：马氏珠母贝 苯并[A]芘 畸形 死亡率 半数致死浓度(LC50) 

分 类 号：X171.5[环境科学与工程—环境科学]