肝细胞癌组织中PEG10、SIAH2的表达及其意义  

Expression of PEG10 and SIAH2 in hepatocellular carcinoma and their clinical implications

作　　者：刘瑶[1] 廖跃光[1] 黄才斌[1] 何晓[2] 

机构地区：[1]赣南医学院第一附属医院消化内科,赣州341000 [2]赣南医学院第一附属医院普外一科,赣州341000

出　　处：《临床与实验病理学杂志》2012年第4期371-373,377,共4页Chinese Journal of Clinical and Experimental Pathology

基　　金：国家自然科学基金(81000908)

摘　　要：目的探讨遗传印记基因PEG10(paternally expressed gene 10,PEG10)与泛素连接酶SIAH2(seven in absentia homologs 2,SIAH2)在原发性肝细胞癌(hepatocellular carcinoma,HCC)组织中的表达及其临床意义。方法采用免疫组化SP法检测50例HCC和相应癌旁组织中PEG10、SIAH2蛋白的表达。结果 (1)PEG10和SIAH2在肝癌组织中的阳性率分别为84%和78%,高于相应癌旁组织中的阳性率8%和14%,差异有统计学意义(P<0.01)。(2)PEG10和SIAH2表达与肿瘤TNM分期有相关性(P<0.01),但与患者的年龄、性别、肿瘤大小、血清HbsAg、AFP水平、是否伴肝硬化无相关性(P>0.05)。(3)HCC中PEG10、SIAH2蛋白表达呈正相关(r=0.42,P<0.01)。结论 PEG10、SIAH2表达与HCC的发生、发展具有明显相关性。Purpose To investigate the expression of paternally expressed gene 10（PEG10） and seven in absentia homologs 2（SIAH2） in hepatocellular carcinoma and to elucidate their clinical significance.Methods The expression of PEG10 and SIAH2 was detected by immunohistoehemistry in 50 cases of hepatocellular carcinoma and adjacent normal tissue（the normal hepatic tissue being at least 5 cm apart from the edge of the tumor）.Results The positive rate was 84% for PEG10 and 78% for SIAH2,significantly higher than adjacent normal hepatic tissue（PEG10： 8%;SIAH2： 14%,P0.01）.Statistic analysis showed that positive immunostaining of PEG10 and SIAH2 protein had association with histology grade（P0.01）,but no association with age,gender,tumor size,serum HBsAg and AFP levels,and cirrhosis of HCC patients（P0.05）.The expression of PEG10 protein was positively correlated with the expression of SIAH2 protein in hepatocellular carcinoma tissues（r=0.42,P0.01）.Conclusion The expression levels of PEG10 and SIAH2 are related to the initiation and progression of hepatocellular carcinoma.

关 键 词：肝肿瘤 肝细胞癌 PEG10 SIAH2 免疫组织化学 

分 类 号：R735.7[医药卫生—肿瘤]