黏液瘤病毒对大鼠体内胶质瘤作用的研究  

Effects of myxoma virus on gliomas of rats models in vivo

作　　者：张秋生[1] 张猛[1] 梁世杰[1] 林恒州[1] 纪涛[1] 李维平[1] 

机构地区：[1]深圳市第二人民医院神经外科,518035

出　　处：《中华实验和临床病毒学杂志》2012年第2期105-107,共3页Chinese Journal of Experimental and Clinical Virology

基　　金：四川省卫生厅课题（070339）

摘　　要：目的探讨黏液瘤病毒对大鼠动物模型体内胶质瘤细胞的作用。方法采用立体定向法向SD大鼠颅内注射c6细胞建立大鼠额叶胶质瘤模型，明确成瘤后，随机分组，以立体定向方法往瘤腔内注射黏液瘤病毒（MV），5-FU，MV＋5-FU及灭活黏液瘤病毒（DV），观察不同组别间大鼠体重、肿瘤大小、GFAP表达、Akt表达情况。结果SD大鼠注射C6细胞后，额叶可见胶质瘤生长。成瘤后瘤腔内注射MV、5-FU及MV＋5-FU，肿瘤的生长较注射DV减慢，并有缩小趋势，GFAP表达较少。MV组及MV＋5-FU组P13k、Akt及roTOR表达较DV组及5-Fu组下降。结论立体定向法注射C6细胞可以建立稳定的胶质瘤模型。MV可以通过调节P13K-Akt-mTOR通路相关基因的表达，从而增加化疗药物对动物模型体内肿瘤细胞的生物学活性。Objective To explore the in vivo effects of myxoma virus （MV） on gliomas of rat model. Methods C6 glioma cells were implanted into the frontal lobe of SD rats using stereotactic methods to establish animal models of glioma. Methods C6 glioma cells were implanted into the frontal lobe of $D rats using stereotactic methods to establish animal models of glioma. Models were divided into 4 groups randomly after tumor growth was affirmed, and MV, 5-FU, MV ＋ 5-FU, and denatured myxoma virus （DV） were implanted into the tumors using stereotactic methods, bodyweight, tumor size, expression of glial fibrillary acidic protein （GFAP）, Akt of each model were observed. Results The gliomas in all SD rats were established successfully. And tumor growth in MV, 5-FU, MV ＋ 5-FU were significantly decreased as compared with DV group after injection, sizes of some tumors were lessened, and GFAP expression decreased in MV, 5-FU and MV ＋5-FU groups. The expression of PI3k, Akt and roTOR were decreased in MV and MV ＋ 5-FU groups. Conclusion C6 glioma SD rat models could be established successfully using stereotactic methods. MV may enhance biological activity of chemotherapeutic drugs on tumor cells of animal models in vivo by regulating some genes of PI3K-Akt-mTOR signal pathway.

关 键 词：神经胶质瘤 黏液瘤病毒 模型 动物 活体研究 

分 类 号：R73[医药卫生—肿瘤]