β肾上腺素能受体兴奋对大鼠急性肝损伤的影响  

Effect of stimulation of β-adrenoceptor on acute liver injury in rats

作　　者：李果[1] 夏锋[1] 吴林[1] 姚超[2] 钱程[2] 别平[1] 

机构地区：[1]第三军医大学西南医院全军肝胆外科研究所,重庆400038 [2]第三军医大学西南医院病理学研究所,重庆400038

出　　处：《第三军医大学学报》2012年第9期809-812,共4页Journal of Third Military Medical University

基　　金：国家自然科学基金(30772095)~~

摘　　要：目的探讨β肾上腺素能受体兴奋对大鼠急性肝损伤的影响。方法使用异丙肾上腺素兴奋β肾上腺素能受体,在不同的用药剂量(1、10、100 mg/kg)和不同的损伤时相点(24、48、72、96 h)以及使用普萘洛尔抑制β肾上腺素能受体兴奋的情况下,检测肝功能指标[谷丙转氨酶(ALT)、谷草转氨酶(AST)、白蛋白(Alb)和总胆红素(TBil)],观察组织病理学变化,探讨β肾上腺素能受体兴奋对大鼠急性肝损伤的影响。结果大鼠肝损伤程度随着异丙肾上腺素剂量的加大而加重[ALT(IU/L):对照组(336.2±140.5),1 mg/kg组(603.7±239.7),10 mg/kg组(838.6±448.5),100 mg/kg组(1 571.5±714.7),P<0.05];在急性肝损伤后第48小时对照组与实验组各项肝功能指标均有统计学差异[ALT(IU/L):(290.8±153.9)、(636.0±385.2),P<0.05;AST(IU/L):(521.0±296.8)、(2 289.1±1 361.5),P<0.05;Alb(g/L):(28.3±1.3)、(25.2±1.5),P<0.05;TBil(μmol/L):(4.1±1.2)、(24.8±16.9),P<0.05];应用普萘洛尔抑制β肾上腺素能受体兴奋,能有效防止大鼠急性肝损伤加重[ALT:兴奋组(606.0±154.9)IU/L,抑制组(210.0±176.1)IU/L,P<0.05]。结论β肾上腺素能受体的兴奋能有效调控大鼠急性肝损伤的进程。Objective To determine the effect of stimulation of β-adrenoceptor by isoprenaline injection on acute liver injury in rats.Methods Effect of intraperitoneal injection of isoprenaline pretreatment to stimulate β-adrenoceptor at different doses（1,10 and 100 mg/kg）,and for different time periods after acute liver injury（24,48,72 and 96 h）,or of propranolol to inhibit β-adrenoceptor,was detected on the liver function index in blood serum and pathological examination.Results With the increase of isoprenaline dose,acute liver injury became more serious（ALT： control group 336.2±140.5 IU/L,1 mg/kg group 603.7±239.7 IU/L,10 mg/kg group 838.6±448.5 IU/L,100 mg/kg group 1571.5±714.7 IU/L,P0.05）.Each index of liver function was statistically different between control group and stimulus group at 48 h after acute liver injury（ALT： 290.8±153.9,636.0±385.2 IU/L,P0.05;AST： 521.0±296.8,2 289.1±1 361.5 IU/L,P0.05;Alb： 28.3±1.3,25.2±1.5 g/L,P0.05;TBil： 4.1±1.2,24.8±16.9 μmol/L,P0.05）.Meanwhile,propranolol inhibited β-adrenoceptor activation,and effectively prevented acute liver injury becoming more serious（ALT： stimulus group 606.0±154.9 IU/L,inhibition group 210.0±176.1 IU/L,P0.05）.Conclusion Stimulation of β-adrenoceptor may effectively regulate the process of acute liver injury in rats.

关 键 词：Β肾上腺素能受体 急性肝损伤 炎症调控 

分 类 号：R363.271[医药卫生—病理学] R575.02[医药卫生—基础医学]