内脏高敏感大鼠结肠Cajal间质细胞C-KIT表达增加  

Increased expression of C-KIT in ICC in colon of rats with visceral hypersensitivity

作　　者：丁瑞峰[1] 王爱鱼[1] 王宏杰[1] 郭元虎[1] 赵鹏程[1] 

机构地区：[1]包头医学院第一附属医院消化科,内蒙古包头014010

出　　处：《基础医学与临床》2012年第5期566-569,共4页Basic and Clinical Medicine

摘　　要：目的检测内脏高敏感大鼠结肠Cajal间质细胞(ICC)C-KIT表达,探讨ICC在内脏高敏感中的作用。方法取出生后8～21 d的Wistar大鼠,实验组每天直肠注射0.6%的冰醋酸0.3～0.5 mL,对照组给予同样剂量的0.9%氯化钠注射液。8周龄时行直肠扩张评估内脏敏感性,记录腹部回缩反射(AWR)为3分时的注水量。取降结肠组织,行C-KIT免疫组化和Western blot检测。结果与对照组相比,实验组大鼠AWR为3分时的注水量明显减少(P<0.05);C-KIT阳性细胞数在实验组大鼠结肠中(23.37±1.88)较对照组(15.33±1.57)明显增加(P<0.05);C-KIT蛋白表达在实验组(134.5±47.5)较对照组(40.6±30.5)明显增强(P<0.01)。结论 ICC增多可能是内脏高敏感的发病机制之一。Objective To investigate the role of ICC in the pathophysiology of visceral hypersensitivity.Methods8 to 21 days after birth,the Wistar rats of the experimental group were injected with recta glacial acetic acid 0.6% 0.3~0.5 mL,the control group were injected the same dose of saline.The visceral sensitivity of 8-week-old rats was evaluated by colorectal distention,and recorded the water injection rate when the score of abdominal withdrawal reflex（AWR） was 3.The distal descending colon was taken to detect for C-KIT immunohistochemisty and Western blot.Results Compared with the control group,the water injection rate of the experimental group showed a significant decrease when the score of AWR was 3;C-KIT masculine cells counting in colon in the experimental group significantly increased;The expression of C-KIT protein in colon in the experimental group significantly increased.Conclusions The increase of ICC of rat with visceral hypersensitivity may be a possible cause of visceral hypersensitivity.

关 键 词：内脏高敏感 结肠 CAJAL间质细胞 原癌基因蛋白质C-KIT 

分 类 号：R363[医药卫生—病理学]