WHBE兔与日本大耳白兔不同生长阶段肠道免疫功能的比较研究  

Comparative Study on Immunological Functions of Intestina Parva Between WHBE Rabbit and Japanese White Rabbit

作　　者：王志远[1,2] 刘月环[2] 余陈欢[2] 应华忠[2] 蔡月琴[1] 张利棕[1] 

机构地区：[1]浙江中医药大学药学院,杭州310053 [2]浙江省医学科学院实验动物中心,杭州310013

出　　处：《实验动物与比较医学》2012年第2期125-128,共4页Laboratory Animal and Comparative Medicine

基　　金：浙江省卫生厅项目（2007QN013）

摘　　要：目的比较WHBE兔与日本大耳白（JW）兔不同生长阶段肠道免疫功能的差异。方法分别选取离乳和2月龄WHBE兔、日本大耳白兔各8只，采用ELISA法，分别测定其血清及小肠匀浆液中分泌型IgA（SIgA）、P物质（SP）、IL-4、IFN-γ、生长抑素（SS）和血管活性肠肽（VIP）的含量水平。结果成年WHBE兔血清及小肠组织sIgA分泌水平、SS、SP含量较离乳WHBE兔明显降低，IFN-γ/IL-4比值明显升高，特别是与成年JW兔在小肠组织IgA分泌水平，SS、IFN．Y和IL-4含量方面具有显著性差异。结论WHBE兔的肠道免疫功能明显低于JW兔，可作为肠易激综合症研究的实验动物。Objective To investigate differences on immunological function of small bowel between WHBE rabbit and Japanese white rabbit. Methods Sixteen WHBE rabbit and Sixteen Japanese white rabbit were used and each group at different stage was 8. Levels of secretory IgA （SIgA）, Substance P （SP）, IL-4, IFN-γ, somatostatin （SS） and Vasoactive Intestinal Peptide （VIP） in serum and small bowel were determined by ELISA. Results The levels of slgA, SS, SP and the ratio of IFN-γ/IL-4 in serum and small bowel of grown WHBE rabbit were much lower than those of weanling WHBE rabbit. In particular, the levels of slgA, SS, IFN-γ and IL-4 in small bowel of grown WHBE rabbit lower than those of grown JW rabbit. Conclusions Immunological function of WHBE Rabbit was much weaker than that of Japanese Rabbit. It may warrant further evaluation as a possible animal for the research of irritable bowel syndrome.

关 键 词：WHBE兔 日本大耳白兔 胃肠道 免疫功能 生长阶段 

分 类 号：R574[医药卫生—消化系统] R-332[医药卫生—内科学]