微小RNA-518b在乳腺浸润性导管癌中的表达及其功能  

Expression pattern and function of microRNA-518b in invasive ductal breast cancer

作　　者：徐丽平[1] 李佳[2] 沈雷[3] 房林[2] 

机构地区：[1]宁波市第一医院普外科,315010 [2]同济大学附属第十人民医院甲乳外科 [3]同济大学附属东方医院

出　　处：《中华实验外科杂志》2012年第5期833-836,共4页Chinese Journal of Experimental Surgery

摘　　要：目的观察miR-518b在乳腺癌中的表达，以及对人乳腺癌细胞株MCF-7增殖、细胞周期、凋亡和侵袭能力的影响。方法运用定量逆转录聚合酶链反应（PCR）检测乳腺癌及正常组织中miR-518b的表达量，结合临床病理资料，分析其异常表达与各病理因素的相关性。并运用噻唑蓝（MTr）比色法了解其增殖，流式细胞仪分析细胞的周期、凋亡，Transwell实验观察其对细胞侵袭能力的影响。结果乳腺癌组织中miR-518b表达水平为2．751585±5．856351，正常组织中为7．334841±9．843424，癌组织中miR-518b表达明显低于正常组织（P〈0．01）。miR-518b在乳腺癌中的表达异常与淋巴结转移相关（P〈0．01），与病理分期、肿瘤大小、原癌基因人类表皮生长因子受体2（HER-2）、雌激素受体（ER）、孕激素受体（PR）、pS3无明显相关性。在48h浓度为150nmoVL时，miR-518b对乳腺癌细胞的抑制作用最明显，抑制率为59．9％。与空对照组和负对照组比较，G0／G1期细胞未见明显增多（75．52±1．78）％，S期无明显减少（9．52±0．91）％，早期凋亡细胞比例未见明显升高（2．60±0．06）％（P〉0．05）。Transwell实验结果显示随着miR-518bmimics浓度的增加，穿出小室膜肿瘤细胞大致呈递减趋势，并与对照组差异明显（P〈0．01）。结论miR-518b在乳腺癌患者中异常表达，癌组织中的表达明显低于正常组织，miR-518b对乳腺癌细胞株的增殖和侵袭能力有明显抑制作用，其在肿瘤发生发展中可能担任着抑癌基因的角色。Objective To explore the expression of miR-518b in invasive ductal breast cancer and analyze its impact on the proliferation, cell cycle, apoptosis and invasion of MCF-7 breast cancer cell line. Methods Twenty pairs of breast cancer and normal tissue specimens were chosen after confirmation of breast invasive ductal carcinoma. We conducted reverse transcription-polymerase chain reaction （RT-PCR） to quantify the expression of miR-518b, and its correlation with clinical data was analyzed by using SPSS software 13.0. To characterize the effect of miR-518b on MCF-7 breast cancer cells, functional assays were respectively employed, including proliferation, cell cycle, apoptosis, and Transwell assays. Results The relative expression of miR-518b in breast cancer specimens was 2. 751 585±5. 856 351, which was significantly lower than 7. 334 841±9. 843 424 of adjacent normal ones （ P 〈 0.01 ）. Aberrant expression of miR-518b was correlated with lymphatic metastasis （P 〈 0.01 ）. On the contrary, no correlation was observed of miR-518b with TNM stage, tumor size, HER-2, estrogen receptor （ER）, progesterone receptor （PR） and 1353 status. Methyl thiazol tetrazolium （MTr） assay revealed that miR-518b mimics significantly inhibited proliferation of breast tumor cells at 48 h after transfection at the concentration of 150 nmol/L with the inhibition rate being 59.9%. In comparison to controls, there was no significant difference in the percentage of G0/G1 phase cells （ 75.52±1.78 ） % , S phase cells （ 9. 52±0. 91 ） % and the proportion of early apoptotic cells （ 2. 60±0. 06） % after miR-518b mimics transfection （ P 〉 0.05 ）. The amount of invaded breast cancer cells in miR-518b transfected groups was significantly different from control groups and declined with an increased miR-518b concentration. Conclusion miR-518b expression is aberrantly expressed in breast cancer. Breast tumor specimens show decreased miR-518b expression compared to their normal counterparts. Considering the

关 键 词：乳腺癌 微小RNA-518b 增殖 细胞周期 

分 类 号：R737.9[医药卫生—肿瘤]