冷冻和重组人肿瘤坏死因子-α在C6大鼠脑胶质瘤治疗中的协同作用  

Cryosurgery and recombinant human tumor necrosis factor-a play synergistic effects on a rat cortex C6 glioma model

作　　者：黄宽明[1] 马廉亭 张力[3] 罗杰 涂汉军[3] 王伦长 

机构地区：[1]南方医科大学,广州510515 [2]广州军区武汉总院神经外科 [3]湖北医药学院附属太和医院神经外科

出　　处：《中华实验外科杂志》2012年第5期907-909,共3页Chinese Journal of Experimental Surgery

基　　金：湖北省教育厅重点科研基金资助项目（2003A007）

摘　　要：目的观察冷冻联合重组人肿瘤坏死因子-a（rhTNF—a）治疗c6大鼠脑胶质瘤治疗的协同作用。方法以原位末端转移酶标记（TUNEL）法检测肿瘤细胞的凋亡，采用免疫组织化学检测增殖细胞核抗原（PCNA）的表达；行核磁共振（MRI）检查，测量肿瘤体积，计算抑瘤率。动态观察各组荷瘤鼠生存期和rhTNF—a对荷瘤鼠的不良反应。结果联合治疗组肿瘤细胞凋亡、PCNA的表达、肿瘤体积和生存期与其他各组比较差异有统计学意义（P〈0．01），且该组对肿瘤生长的抑制率（60．38％；89．48％）明显大于冷冻组（42．69％；66．31％）和TNF—a组（21．68％；49．01％），且大于理论抑瘤率（82．82％）。rhTNF-a对实验动物有不良反应。结论冷冻和rhTNF-a对c6联用的抗肿瘤作用进一步加强，具有协同作用。Objective To observe the synergistic effects of freeze combination with recombinant human tumor necrosis factor-a （rhTNF-a） on the treatmemt of rat C6 brain glioma. Methods TdT-medi- ated dUTP nick end labeling （TUNEL） was applied to detect apoptosis. Proliferating cell nuclear antigen （PCNA） expression in tumor cell was examined by SP immunohistoehemical staining. Meanwhile, the tumor volume were measured by MRI and tumor growth inhibition rate was acquired. The survival period of rats and the poison side effect of rhTNF-a were dynamically observed. Results After comparing the combined group with the other three groups, the statistical significance was exhibited about PCNA expression, tumor volume and survival period （ P 〈0. 01 ）. The inhibition rate of tumor growth in combined group （60. 38% ; 89. 48% ） was significantly higher than in cryotherapy group （42. 69% ; 66. 31% ） and in rhTNF-ct group （21.68% ; 49. 01% ）, and even larger than theories inhibition rate （55.11% ; 82. 82% ） of tumor growth . At the same time, the certain poison side effect to the experiment animal was caused by rhTNF-a. Conclusion The combination of freeze and rhTNF-a could strengthen efficiency of antitumor and had synergie effects in the treatment of glioma.

关 键 词：神经胶质瘤 治疗 冷冻疗法 重组人肿瘤坏死因子-a 协同作用 

分 类 号：R739.4[医药卫生—肿瘤]