马铃薯StKN1基因的cDNA克隆与分析  

cDNA Cloning and Sequence Analysis of StKN1 from Solanum tuberosum

作　　者：徐平丽[1,2] 张传坤[2,3] 单雷[1,2] 李新国[1,2] 

机构地区：[1]山东省农业科学院高新技术研究中心,山东济南250100 [2]山东省作物与畜禽品种改良生物技术重点开放实验室,山东济南250100 [3]山东省农业科学院行政处,山东济南250100

出　　处：《山东农业科学》2012年第4期1-4,共4页Shandong Agricultural Sciences

基　　金：山东省自然科学基金(ZR2009ZD007);山东省农业科学院青年基金(2007YQN003)

摘　　要：植物同源异型枢基因(Homeobox gene,HBG)广泛存在,是一类重要的转录因子编码基因,在生物进化中具有很强的保守性。利用GenBank登录的拟南芥、苜蓿、豌豆、烟草、番茄等同源异型枢基因保守区序列设计简并引物,通过RT-PCR技术,从马铃薯萌动的幼芽中获得了一个homeobox的cDNA基因,命名为StKN1,GenBank登录号为DQ494855.1。该基因片段长971 bp,经序列分析比对,与其他植物的HBG具有很高的序列相似性。系统进化分析将StKN1基因归入homeobox KNOX基因家族。Plant homeobox genes are an important class ot transcnption tactor encoding genes involvead in plant individual development. Plant homeobox genes and its encoding protein have highly conservative struc-ture. After alignment of plant homeobox genes of Arabidopsis thaliana, Medicago truncatula, Pisum sativum, Nicotiana tabacum and then submitted on GenBank, a highly conserved sequence was selected as target frag- ment for cloning. The homeobox gene fragment was amplified by RT - PCR from eDNA of Solanum tuberosum bud, named as StKN1 with the accession number as DQ494855.1. The sequence analysis showed that the StKN1 fragment was 971 bp in length and had higher sequence similarity with the other plants＇ HBG. The ev-olutionary analysis showed that StKN1 was a member of homeobox KNOX gene familly.

关 键 词：马铃薯 StKN1 CDNA 基因克隆 序列分析 

分 类 号：Q785[生物学—分子生物学]