内镜下卢戈氏液染色与p53蛋白检测诊断早期食管癌的体外试验  

In vitro study of iodine staining and p53 oncoprotein for early esophageal cancer

作　　者：魏子白[1] 李玉翠[1] 田步升[2] 王丽冰[3] 王敬弘[2] 

机构地区：[1]长治医学院附属和平医院消化内科,046000 [2]长治医学院附属和平医院心胸外科,046000 [3]长治医学院附属和平医院病理科,046000

出　　处：《中华消化内镜杂志》2000年第1期20-22,共3页Chinese Journal of Digestive Endoscopy

摘　　要：目的研究内镜下卢戈氏液染色和活组织 p53检测诊断食管早期癌和表浅癌的可行性。方法将97例食管癌手术标本先用卢戈氏液染色 ,然后对粘膜不染色区行 p53蛋白免疫组化染色。结果在97例食管癌手术标本中 ,共有63处直径大于2mm的粘膜不染色区 ,其中正常粘膜2处 ,单纯增生7处 ,不典型增生42处 ,原位癌1处 ,鳞状细胞癌11处 ;p53蛋白的阳性表达主要发生在重度不典型(42.3 %)和鳞状细胞癌(45.5 %)的细胞核内 ,而在正常粘膜、单纯增生和轻～中度不典型增生组织中几乎无表达(p<0.05)。Objective The feasibility of Lugol’s iodine staining in association with p53 oncoprotein test to detect early esophageal cancer through endoscopy was explored .Methods Lugol’s iodine was sprayed on resected esophageal specimens ( n=97 ) . Biopsies taken from areas > 2 mm in diameter unstained by iodine were prepared immunohistochemically for p53 oncoprotein study.Results There were 63 areas unstained by iodine including dysplasias ( n=42 ) carcinoma in situ ( n=1 ) and squamous cell carcinomas ( n=11 ) . Expression of p53 was more frequently found in severe dysplasia and carcinoma than in normal mucosa or mild to moderate dysplasia ( 45.5%, P < 0.05 ) .Conclusion This modified method seemed feasible to detect early esophageal cancer via endoscopy .

关 键 词：食管肿瘤 P53蛋白 卢戈氏液染色 内窥镜 

分 类 号：R735.1[医药卫生—肿瘤]