老年性痴呆患者IgG合成率及脑脊液Tau β-淀粉样蛋白测定分析  

Detection of Tau proteins β－Amyloid protein and IgG synthesis in Alzheimer's disease

作　　者：王磊 袁锦楣[1] 郝洪军[1] 李海峰[1] 李静[1] 金弘敏[2] 冀成君[2] 牛俊英 

机构地区：[1]北京医科大学第一医院神经科免疫室,北京100034 [2]北京回龙观医院老年科 [3]解放军262医院神经内科,北京100084

出　　处：《河南实用神经疾病杂志》2000年第1期10-12,共3页Henan Journal of Practical Nervous Diseases

摘　　要：目的：测定Alzhuner病（AD）部分实验室指标，探讨其与临床的关系。方法：采用火箭电泳法（RIE），测定IgG和白蛋白含量，计算IgG鞘内合成率；运用酶联免疫吸附法（ELISA），测定脑脊液Tau和β－淀粉样蛋白（β－AP）的含量。结果：血清、脑脊液中白蛋白水平正常组最高，血清、脑脊液IgG水平AD组最高。但三级间均存在较大重叠。老年人脑脊液IgG鞘内合成率增高比例较大，无显著性差异。AD、MID患者脑存液Tau蛋白，β－AP含量均明显高于对照组，差别具有统计意义（P＜0．001），但二者差别无统计意义（P＞0．05）；结论：血清、脑存液中白蛋白水平和IgG水平在三组间无显著性差异；Tau蛋白和β－AP含量在AD患者脑誉液明显升高，在MID组亦增高，故单纯测定二者难以鉴别AD和MID，但可作为AD的实验室表现。在IgG鞘内合成率异常者中，Tau蛋白含量增高而β－AP含量降低者以AD最多、至于这样综合分析是否可为AD诊断提供实验室依据沿有待加大样本量进一步探讨。Objective To explore a pecific experimental measurement of Alzheimer 's disease (AD). Methods: The levels of IgG and albumin of serum and cerebrospinal fluld was determined in 20 AD patients, 13 MID patients and 30 normal controls with RIE; calculate the 24 hours IgG synthe sis. The levels of tau and β- Amyloid protein in CSF was determined with ABC - ELISA. Results: The levels of albumin and IgG were overlap in AD,MID patients and normal cormal controls . There was no significent difference the three groups (P>0.05). There was no correlation between the levels of IgG synthesis and AD, The CSF- Tau and β- Amyloidprotein levels were aiedcently elevated in AD and MID patients, There are no significent difference between the two groups (P > 0.05). Conchusions: So simple detection is not a specific experimental diagnosis of AD, but it may be helpful to show the Blood - brain - Bartier damage in aged and to the diagnosis of AD when asssociated with other clinical investigations.

关 键 词：IGG合成率 老年性痴呆 TAU蛋白 Β-AP 痴呆 

分 类 号：R749.160.4[医药卫生—神经病学与精神病学]