DNA甲基化在热休克诱导细胞周期素D1表达水平降低中的转录调控机制  

Mechanism of DNA methylation in the regulation of heat shock-induced transcriptional inhibition of cyclin D1 expression

作　　者：郭志义[1] 郝小惠[1] 高雯雯[2] 姜雪莲[2] 赵丹丹[2] 陈远[2] 杨方[1] 

机构地区：[1]河北联合大学医学实验研究中心,河北唐山063000 [2]河北联合大学生命科学学院,河北唐山063000

出　　处：《广东医学》2012年第7期894-896,共3页Guangdong Medical Journal

基　　金：国家自然科学基金资助项目(编号:31050010);河北省高等学校科学研究计划项目(编号:2010154)

摘　　要：目的研究DNA甲基化在热休克诱导的人细胞周期素D1基因启动子活性下降调控中的作用。方法 DNA甲基化通过甲基化特异性PCR方法检测。甲基化位点突变采用Stratagene公司定点突变试剂盒。甲基化位点的作用通过报告基因技术分析启动子活性确认。结果热休克处理可能造成细胞周期素D1基因启动子+65/67位点发生DNA甲基化,而突变该位点逆转了由报告基因活性分析研究的热休克效应。结论启动子+65/67位点的DNA甲基化可能调控细胞周期素D1的热休克效应。Objective To investigate the role of DNA methylation in heat shock-induced inhibition of human cyclin D1 expression.Methods The DNA methylation status and mutatan sites were detected by Methylmion Specific PCR（MSP） and Stratagene kit,respectively.The effects of the methylation sites were evaluated by reporter gene analysis.Results Suggest that the ＋65/67 sites of human cyclin D1 promoter were methylated under heat shock treatment.The heat shock response evaluated by reporter gene analysis was inverted when the putative methylation sites was mutated.Conclusion The heat shock-induced inhibition of cyclin D1 transcription involves the methylation of ＋65/67 CpG sites.

关 键 词：DNA甲基化 甲基化特异性PCR 热休克 细胞周期素D1 

分 类 号：R737.33[医药卫生—肿瘤]