eIF4E基因下调对人乳腺癌Bcap37细胞凋亡的影响  

Effects of eIF4E Gene Down-regulation on Apoptosis of Human Breast Cancer Cell Line Bcap37

作　　者：林宇[1] 许惠玉[1] 邹宇[2] 崔红霞[1] 刘吉成[1] 

机构地区：[1]齐齐哈尔医学院,161006 [2]黑龙江中医药大学,哈尔滨150040

出　　处：《医学研究杂志》2012年第4期41-43,共3页Journal of Medical Research

基　　金：国家自然科学基金资助项目(30973902)

摘　　要：目的应用化学合成的siRNA干扰乳腺癌Bcap37细胞真核细胞翻译起始因子4E(eukaryotic translation initiationfactor 4E,eIF4E)的表达,探讨其对乳腺癌细胞凋亡和生长周期的影响。方法化学合成eIF4E基因的siRNA,转染人乳腺癌Bcap37细胞,采用Western blotting法及RT-PCR法检测转染前后Bcap37细胞中eIF4E蛋白和mRNA表达;应用流式细胞技术检测转染前后细胞周期的变化;AO/EB双染色法检测细胞凋亡;酶标仪检测caspase-3活性。结果 RT-PCR与Western blot-ting法检测结果显示,转染eIF4E-siRNA-3#组细胞eIF4E基因的mRNA和蛋白表达均明显下降(P<0.01);eIF4E-siRNA转染组G1期细胞比例显著增加,S期细胞比例相对减少,差异显著(P<0.05);转染eIF4E-siRNA可诱导细胞凋亡,caspase-3活性亦增强。结论 eIF4E-siRNA可下调eIF4E基因在乳腺癌Bcap37细胞中的表达水平,诱导细胞凋亡。Objective To investigate the effect of down - regulation of eIF4E on the apoptosis and cell cycle of human breast cancer Bcap37 cells. Methods The siRNA of eIFdE by chemical systhesis was transfeeted into breast cancer Bcap37 cells. The expressions of elF4E mRNA and protein were examined by RT -PCR and Western blotting. AO/EB doubly staining and microplate spectrophotometer were used to detect the apoptosis and activity of caspase - 3. Results The expression of eIF4E mRNA and protein were all decreased markedly after transfection with eIF4E - siRNA - 3#. The results of cell cycle detection showed that the percentage of Bcap37 cells in the Gl phase obviously increased, while the percentage of Bcap37 cells in the S phase significantly reduced（ P 〈 0.05 ）. It also can induce ap- optosis of cells and increase the activity of caspase - 3. Conclusion Transfection with elF4E - siRNA - 3# can down - regulate eIF4E gene expression in human breast cancer Bcap37 cells and induce apoptosis of cells.

关 键 词：真核细胞翻译起始因子 细胞凋亡 BCAP37 

分 类 号：R737.902[医药卫生—肿瘤]