灯盏花素对糖尿病大鼠肾皮质糖基化终产物受体mRNA表达的影响  

Effects of Erigeronbreviscapus on Receptor for Advanced Glycation End Products(RAGE) mRNA in Renal Cortex of Diabetic Rats

作　　者：孙洁敏[1] 马晓兰[2] 沈建国[2] 姚美芬[2] 李璐璐[2] 李琦[2] 

机构地区：[1]浙江医院内分泌科,310013 [2]浙江大学医学院附属第一医院内分泌科

出　　处：《医学研究杂志》2012年第4期131-133,共3页Journal of Medical Research

摘　　要：目的探讨灯盏花素对链脲佐菌素(STZ)诱导的糖尿病大鼠肾皮质RAGE表达水平的影响。方法雄性SD大鼠27只,随机分为3组。STZ腹腔注射建立糖尿病模型后,糖尿病灯盏花素治疗组(DD组)每日给予灯盏花素[20mg/(kg.d)]灌胃,8周后取肾脏组织行半定量RT-PCR检测RAGE mRNA的表达水平,与正常对照组(C组),糖尿病安慰剂组(D组)比较。结果成模8周时,糖尿病大鼠的体重、血糖与正常组相比有显著性差异(P<0.01),DD组与D组的血糖及体重无显著性差异(P>0.05);DD组、D组和C组的RAGE OD值/β-actin OD值分别为0.452±0.208,0.769±0.104,0.218±0.032,各组间RAGE表达有显著性差异(P<0.01)。结论糖尿病大鼠肾皮质RAGE mRNA表达明显高于正常对照组。灯盏花素能下调肾皮质RAGE的表达水平,对糖尿病肾病具有一定治疗作用。Objective To determine whether erigeronbreviscapus affects expression of RAGE mRNA in renal cortex of streptozotocin - induced diabetic rats. Methods We induced diabetic rats by an intraperitoneal injection of streptozotocin （STZ） in SD rats. The criterion of diabetes diagnosis was the consecutive blood glucose level≥16.7mmol/L. Totally 27 male SD rats were randomly divided into 3 groups：diabetes adding erigeronbreviscapus group （DD group, intragastric administration erigeronbreviscapus 20rag/（ kg· d） and diabetes group （D group） and normal control group （C group）. The body weight and blood glucose were measured every two weeks. Totally 8 weeks later, all rats were killed and the expression of RAGE was semiquantified in renal cortex by reverse transcription - polymerase chain reaction （ RT - PCR） , and renal morphology was evaluated respectively. Results The RAGE expression was increased in renal cortex of diabetic rats. RAGE/[5 -actin ratio of diabetic group was significantly higher than that of the control （P 〈0. 01 . After treatment with erigeronbreviscapus, RAGE expression decreased significantly. Conclusion The high expression of RAGE may pamcipate in the renal damage of diabetic rats. Erigeronbreviscapus has no effect on the level of serum glucose, but significantly down - regulate RAGE expression in renal. It may have some renal protective effect on diabetic nephropathy, partly through inhibition of excessive expression of RAGE in renal cortex.

关 键 词：STZ 灯盏花素 晚期糖基化终产物受体 

分 类 号：R36[医药卫生—病理学]