白血病细胞多药耐药基因表达与化疗耐药关系的研究  

Study of the relationship between mdrl gene expression and resistance to chemotherapy in leukemia

作　　者：吴培红[1] 孔宪明[1] 缪金明[1] 邵念贤[1] 方智雯[1] 欧阳仁荣[1] 

机构地区：[1]上海第二医科大学附属仁济医院血液科

出　　处：《上海医学》2000年第1期34-37,共4页Shanghai Medical Journal

摘　　要：目的　研究ｍｄｒｌ 基因表达与临床化疗的关系。方法　选择４４ 例白血病患者和５ 例正常对照者，运用ＲＴＰＣＲ方法检测ｍｄｒｌ 基因表达，同时评估患者对化疗的反应。结果　白血病患者初治组ｍｄｒｌ 阳性表达率为７８．９％ ，缓解组为５５ ．６ ％ ，难治复发组为９３ ．３ ％ ，对照组无一人表达；ｍｄｒｌ 阴性及低度表达者中有８２ ．４ ％的人达到ＣＲ，而ｍｄｒｌ 中、高度表达者中仅３７．０％ 的人达到ＣＲ（ Ｐ＝ ０ ．００３） 。结论　ｍｄｒｌ 基因表达增高与化疗耐药有关，可作为监测病情和判断预后的一个辅助指标。Objective To study the relationship between mdrl gene expression and clinical treatment. Methods We analysed forty four leukemic patients and five normal controls by RT PCR to determine mdrl gene expression. Results 78.9% of newly diagnosed patients, 55.6% of remission patients and 93.3% of refractory & relapsed pateints expressed mdrl gene, while none of the normal controls had this gene expression. 82.4% of mdrl negative and low expressed patients reached CR, while CR rate was only 34% in mdrl intermediate and highly expressed patients ( P =0.003).Conclusion These data indicate that high level of mdrl gene expression may lead to resistance of tumor cells to chemotherapy. So the mdrl gene expression can serve as a supplementary marker to monitor the patients' condition and determine the prognosis of the disease. (Shanghai Med J, 2000,23:34 37)

关 键 词：多药耐药基因 白血病 耐药性 RT-PCR 

分 类 号：R730.5[医药卫生—肿瘤]