HPV-18病毒E2与E6在宫颈病变中的表达与意义  

Expressions of human papillomavirus-18 E2 and E6 in cervical lesions

作　　者：黄卓敏[1] 王楚莹[1] 古衍[1] 姚吉龙[1] 谢建生[2] 

机构地区：[1]南方医科大学附属深圳市妇幼保健院妇科,518028 [2]南方医科大学附属深圳市妇幼保健院中心实验室,518028

出　　处：《国际医药卫生导报》2012年第7期896-899,共4页International Medicine and Health Guidance News

基　　金：深圳市科技计划立项项目（201003104;201102091）

摘　　要：目的探讨HPV—18病毒E2与E6基因在宫颈病变组织中的表达及其在宫颈癌变过程中的作用。方法采用逆转录聚合酶链反应技术，检测HPV-18阳性的宫颈炎15例、CINI19例、CINⅡ17例、CINⅢ20例、宫颈浸润癌24例的标本中E2及E6基因的表达，比较两者在各组中的表达差异。结果HPV-18E2在宫颈炎、CINI、CINⅡ、CINⅢ和宫颈浸润癌的阳性表达率分别为93．3％、94．7％、58．8％、20．0％及0％；E6在上述各组的阳性表达率分别为6．7％、5．3％、41．2％、85．O％及100％；E2和E6在宫颈炎和CINI的表达差异无显著性（P〉0．05）；E2和E6的表达在CINI、CINII、CINⅢ和宫颈浸润癌组间的比较，差异有显著意义（P〈0．05）；E6和E2的表达呈负相关（r=-0．728，P〈0．01）。结论HPV-18E2的表达缺失和E6的过度表达，可能是HPV诱导宫颈细胞恶性转化的关键环节，在宫颈癌的演变过程中起重要作用。检测HPV-18E2与E6的表达对早期评估CIN的预后可能有一定的参考价值。Objective To investigate the expressions of human papillomavirus-18 E2 and E6 oncogene in cervical lesions and its correlation with cervical carcinogenesis. Methods The expressions of HPV-18 E2 and E6 gene in the tissues from 15 cases of cervicitis, 19 cases ofCIN I , 17 cases ofCIN Ⅱ , 20 cases of CIN Ⅲ, and 24 cases of invasion cervical carcinoma with HPV- 18 infection were detected by reverse transcription polymerase chain reaction. Results The positive rate of E2 gene expression in groups of cervicitis, CIN I , CIN Ⅱ , CIN Ⅲ and invasion cervical carcinoma were 93.3 %, 94.7 %, 58. 8%, 20.0% and 0% respectively, and that of E6 gene were 6.7%, 5.3%, 41.2%, 85.0% and 100% respectively. No significant differences of E2 and E6 gene expressions were found between groups of cervicitis and CIN Ⅰ （ P〉 0.05 ）. However, there were significant differences between any two groups of CIN I , CIN Ⅱ , CIN Ⅲ and invasion cervical carcinoma （ P 〈 0.05 ）. The expression of E6 gene was inversely correlated with expression of E2 gene （ r= -0.728, P 〈 0.01 ）. Conclusions Deletion of HPV-18 E2 and overexpression of E6 which has been regarded as a key step in malignant transformation of cervical cells inducted by HPV may play an important role in cervical carcinogenesis. Detection of levels of HPV- 18 E2 and E6 expression might be clinically valuable in the prediction of cervical carcinogenesis and the evaluation of prognosis in CIN.

关 键 词：人乳头瘤病毒18 E2 E6 宫颈上皮内瘤变 宫颈癌 

分 类 号：R737[医药卫生—肿瘤]