癌相关纤维母细胞对胃癌细胞增殖、凋亡、迁移和侵袭活性的影响  

The effects of cancer-associated fibroblasts on proliferation,apoptosis,migration and invasion of human gastric cancer cells

作　　者：单丽辉[1] 张思 翟丽丽[3] 齐蕾[1] 柴翠翠[1] 韩伟[1] 王立峰[1] 

机构地区：[1]哈尔滨医科大学附属第一临床医院病理科,哈尔滨150001 [2]哈尔滨市第五医院骨五病房 [3]齐齐哈尔市第一医院

出　　处：《实用肿瘤学杂志》2012年第2期97-101,共5页Practical Oncology Journal

基　　金：黑龙江省自然科学基金项目（D201103）

摘　　要：目的 探讨癌相关纤维母细胞（CAFs）对胃癌细胞增殖、凋亡、迁移和侵袭活性的影响,为胃癌的合理有效治疗提供新的思路和作用靶点.方法 （1）原代培养正常纤维母细胞（NFs）和CAFs,用CCK8实验和流式细胞仪检测间接共培养后,NFs和CAFs对MGC-803细胞增殖和凋亡能力的影响;（2）通过transwell共培养小室建立MGC-803与CAFs相互作用的体外模型,分析CAFs对MGC-803迁移和侵袭能力的影响.结果 （1）与NFs相比,CAFs在体外传代培养过程中,仍高表达波形蛋白（Vimentin）、纤维母细胞活化蛋白（FAP）;（2）与NFs组相比,CAFs促进MGC-803细胞增殖、迁移和侵袭,但是抑制其凋亡.结论 CAFs在胃癌的生长、迁移和侵袭等过程中起着重要的作用,并且可能成为胃癌治疗的新靶点.Objective We aim to investigate the effects of CAFs on proliferation, apoptosis, migration and invasion of human gastric adenocarcinoma. CAFs can be as a novel target for the treatment of gastric cancer. Methods （ 1 ） The crosstalk between the two types of fibroblasts and the gastric cancer cell line MGC - 803 was evaluated using an in vitro indirectly co-culture model. Cell proliferation and apoptosis assays were performed u- sing a Cell Counting Kit - 8 and flow cytometry. （2） The interaction model between primary gastric NFs/CAFs and gastric cancer cell line MGC - 803 was established by transwell chamber to observe the effect of migration and invasion in MGC - 803. Results （ 1 ） We found that CAFs expressed higher levels of Vimentin and fibroblast activation protein（FAP） than did NFs. （2）CAFs may promoted proliferation, migration（ 4.08 -fold）and invasion （5.12 - fold） to a greater extent than did NFs in MGC - 803 （ P 〈 0.05 ）, but inhibited the apoptosis of gastric cancer cell line MGC - 803 （ P 〈 0.05 ）. Conclusion Taken together, these findings indicate that CAFs may play a vital role in gastric cancer growth ,migration and invasion. Targeting CAFs as a therapeutic strategy for the treat- ment of gastric cancer is an intriguing concept that warrants further study.

关 键 词：胃癌 癌相关纤维母细胞 肿瘤-间质相互作用 肿瘤微环境 

分 类 号：R735.2[医药卫生—肿瘤]