红花多糖抑制PI3K/Akt信号通路诱导人胃癌细胞凋亡的研究  

Safflower polysaccharides inhibit PI3K/Akt signaling pathway induces apoptosis of human gastric cancer cells

作　　者：陶冀[1] 黎清炜[1] 石学魁[2] 梁颖[3] 王亚贤[3] 

机构地区：[1]哈尔滨医科大学附属第三医院肿瘤内科,哈尔滨150081 [2]牡丹江医学院微生物与免疫学教研室 [3]黑龙江中医药大学微生物与免疫学教研室

出　　处：《实用肿瘤学杂志》2012年第2期119-124,共6页Practical Oncology Journal

基　　金：黑龙江省教育厅资助课题（11531119）

摘　　要：目的 探讨红花多糖通过抑制PI3K/Akt信号通路调控胃癌细胞凋亡的机制.方法 MTT比色法观察SPS对人胃癌SGC-7901细胞体外增殖的抑制作用,流式细胞仪（FCM）分析细胞凋亡,实时荧光定量RT-PCR法和Western Blot法检测蛋白激酶B（Akt）基因及蛋白的表达情况.结果 红花多糖在一定范围内以剂量依赖方式和时间依赖方式抑制SGC-7901胃癌细胞生长.流式细胞仪检测,SGC-7901细胞经红花多糖处理24h,其早期凋亡率、细胞坏死或晚期凋亡率显著增加,呈现明显的剂量依赖性.Real-time PCR和Western Blot检测发现,SPS处理的细胞Akt基因及蛋白表达量明显下降.结论 红花多糖对人胃癌SGC-7901细胞体外增殖具有明显的抑制作用,该抑制作用具有一定的时间依赖性和剂量依赖性;红花多糖能够下调Akt mRNA表达,降低Akt和p-Akt蛋白的表达量,抑制Akt通路发挥抗肿瘤作用.Objective To investigate how the safflower polysaceharides （SPS）regulate the apoptosis of human gastric cancer cell by inhibiting the FI3K/Akt signaling pathway. Methods We used MTT colorimetrie method to observe the proliferation of human gastric cancer cell （ SGC - 790） in vitro and used flow eytometry （FCM） to analysis the apoptosis of the SGC -790 Cell. The expression of the protein kinase B（Akt） was detected by real - time quantitative RT - PCR and Western Blot, respectirely. Results SPS strongly inhibited gastric car- cinoma cell line SGC -7901 to proliferate, and this inhibition relied on time and dosage conspicuously. Analyzed with flow cytometry, SGC -7901 cell treated with SPS for 24 hours showed increased percentage of early time ap- optosis,cell necrosis and late time apoptosis, and took on dose dependence. Detected by real - time PCtt and Western blot,expression of gene and protein of BCL -2 and Akt decreased and that of gene and protein of BAX increased in the cells treated with SPS. Conclusion SPS can inhibit human gastric carcinoma cell to proliferate, and this inhibition has time and dose dependence. SPS can cut down the expression of gene and protein of Akt. SPS restrain Akt pathways which may play antitumor function.

关 键 词：红花多糖 PI3K/AKT 凋亡 胃癌 

分 类 号：R735.2[医药卫生—肿瘤]