CXCR7对HeLa细胞增殖、粘附和侵袭能力的影响  

The role of CXCR7 on the proliferation,adhesion and invasion of HeLa cells

作　　者：孙炜[1] 蔡绍皙[1] 晏小清[1] 陈斯佳[1] 宋振[1] 叶群芳[1] 

机构地区：[1]重庆大学生物工程学院生物流变科学与技术教育部重点实验室,重庆400030

出　　处：《中国科学技术大学学报》2012年第4期311-317,共7页JUSTC

基　　金：国家自然科学基金(11172339);国家自然科学基金青年科学基金(81000067);"211工程"三期建设项目(S-09104);重庆大学大型仪器设备开放基金资助

摘　　要：基质细胞衍生因子-1(SDF-1)在肿瘤侵袭、转移过程中起着重要的调控作用.此前认为SDF-1是通过其唯一受体CXCR4来起作用.近年来发现SDF-1还有另一作用受体——CXCR7,SDF-1/CXCR7在部分肿瘤侵袭转移过程中起重要作用,但其在宫颈癌HeLa细胞中的作用目前尚未明确.通过Western blotting检测HeLa细胞中CXCR4和CXCR7的表达,阻断CXCR4或CXCR7后,通过MTT法评价细胞增殖能力,细胞粘附实验评价细胞粘附能力,Transwell实验评价细胞侵袭能力.结果表明,CXCR4和CXCR7在HeLa细胞中表达.阻断CXCR4或CXCR7后,SDF-1诱导的HeLa细胞增殖、侵袭和与内皮细胞的粘附能力均被阻断.结果提示CXCR7在SDF-1诱导HeLa细胞增殖、侵袭和与内皮细胞的粘附过程中起着重要作用,将有望成为治疗宫颈癌转移的新靶点.Stromal cell-derived factor 1 （SDF-1） is a principal regulator ot~ tumor invasion and metastasis. SDF-1 had been considered to mediate biological process through its unique receptor CXC chemokine receptor 4 （CXCR4） for many years. Recent studies reported that SDF-1 was also a ligand o{ a novel chemokine receptor-CXC chemokine receptor 7（CXCR7）. It was confirmed that CXCR7 plays an important role in invasion and metastasis of several types of tumor induced by SDF-1. However, its role in cervical cancer is still unclear. In the present study, the expressions of CXCR4 and CXCR7 on HeLa cells weredetected by Western blotting and the effects of CXCR4 and CXCR7 on cell behaviors were tested by blocking with their antagonists, respectively. Cell proliferation, cell invasion and cell adhesion were evaluated by MTT, Transwell assay, adhesion assay, respectively. Results reveal that both CXCR4 and CXCR7 are expressed on HeLa cells. The proliferation, invasion and adhesion of HeLa cells induced by SDF＇-I are inhibited by CXCR4 blockage or CXCR7 blockage. These results suggest that CXCR7 can mediate proliferation, invasion and adhesion of HeLa cells induced by SDF1, which indicates that CXCR7 is a potential target for cervical cancer therapy.

关 键 词：HELA细胞 CXCR7 细胞增殖 细胞粘附 细胞侵袭 

分 类 号：R737.33[医药卫生—肿瘤] R73-37[医药卫生—临床医学]